Glasgow/Wetlab/Gels

From 2007.igem.org

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Revision as of 12:33, 28 August 2007

Glasgow Main Page | Back To Wetlab Log


Gels

Gels are labelled with numbers for rows and letters for columns.

Date and Reference Description Key Gel
Fri 10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. 1.(*m*)

2.(*m*)
3.(*s*)
4.(*s*)
5.(*s*)
6.(*s*)
7.neg control
8.neg control
9.neg control

10thAug gel 1.JPG
Fri 10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. 1.(*s*)

2.(*s*)
3.neg control
4.DntR
5.DntR
6.Pu
7.Pu
8.neg control
9.neg control
10.neg control
11.neg control

10thAug gel 2.JPG
Mon 13th Aug 2007 (4) PCR of genes (*a→g*), (*a→d*), and (*d→g*). 1A:(*a→g*)

1B:(*a→g*)
1C:(*a→g*)
2A:(*a→g*)
2B:(*a→d*)
2C:(*d→g*)

2007-08-13 12hr 43min.JPG
Fri 17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. 17thAug SDM1 PstI digest gel 1.JPG
Fri 17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. 17thAug SDM1 PstI digest gel 2.JPG