Glasgow/Wetlab/Gels

From 2007.igem.org

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|[[Glasgow/Wetlab/Week8#Friday 24th August 2007|24th Aug 2007]]
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|[[Glasgow/Wetlab/Week8#Friday 24th August 2007|24th Aug 2007 (1)]]
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|These are the miniprepped plasmids from XylR+Pr in TOPO vector. The seven colonies gave the right size product from colony PCR but failed to give right size bands after miniprepping. Wait for sequencing results.
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|'''1.-7.'''colonies from XylR+Pr in TOPO transformations
|[[Image:2007-08-24 12hr 54min Maija.JPG|200px]]
|[[Image:2007-08-24 12hr 54min Maija.JPG|200px]]
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Revision as of 13:40, 29 August 2007

Glasgow Main Page | Back To Wetlab Log


Contents

Gels

Friday 10th August 2007

Date and Reference Description Key Gel
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. 1.(*m*)

2.(*m*)
3.(*s*)
4.(*s*)
5.(*s*)
6.(*s*)
7.neg control
8.neg control
9.neg control

10thAug gel 1.JPG
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. 1.(*s*)

2.(*s*)
3.neg control
4.DntR
5.DntR
6.Pu
7.Pu
8.neg control
9.neg control
10.neg control
11.neg control

10thAug gel 2.JPG

Monday 13th August 2007

Date and Reference Description Key Gel
13th Aug 2007 (4) PCR of genes (*a→g*), (*a→d*), and (*d→g*). 1A.(*a→g*)

1B.(*a→g*)
1C.(*a→g*)
2A.(*a→g*)
2B.(*a→d*)
2C.(*d→g*)

2007-08-13 12hr 43min.JPG

Friday 17th August 2007

Date and Reference Description Key Gel
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. 1.-3.(*m*)+4/6B
(PstI site mutated)

4.-6.(*m*)+3/20G
(PstI site mutated)
7.-9.(*s*)+4/6B
(1st PstI site mutated)
10.-12.(*s*)+3/20G
(2nd PstI site mutated)
13.-15.(*s*)+4/6B
(2nd PstI site mutated)
16.(*s*)+3/20G
(1st PstI site mutated)

17thAug SDM1 PstI digest gel 1.JPG
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. 1.-2.(*s*)+3/20G
(1st PstI site mutated)

3.(*m*)+4/6B
(Before mutagenesis)
4.(*m*)+3/20G
(Before mutagenesis)
5.(*s*)+4/6B
(Before mutagenesis)
6.(*s*)+3/20G
(Before mutagenesis)

17thAug SDM1 PstI digest gel 2.JPG

Thursday 23rd August 2007

Date and Reference Description Key Gel
23rd Aug 2007 (*s*) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. 1.-5.(*s*)+4/6B

6.-16.(*s*)+3/20G

Maija's Gel taken by Scott.JPG
23rd Aug 2007 PstI digested (*s*) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. 1.-5.(*s*)+4/6B

6.-16.(*s*)+3/20G

23rdAug 13hr 46min Maija.JPG
23rd Aug 2007 Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1. 1.-7.colonies from the plate Gel for Maija 1.JPG
23rd Aug 2007 Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2. 8.-12.colonies from the plate

13.neg control

Gel for Maija 2.JPG


Thursday 24th August 2007

Date and Reference Description Key Gel
24th Aug 2007 (1) These are the miniprepped plasmids from XylR+Pr in TOPO vector. The seven colonies gave the right size product from colony PCR but failed to give right size bands after miniprepping. Wait for sequencing results. 1.-7.colonies from XylR+Pr in TOPO transformations 2007-08-24 12hr 54min Maija.JPG