Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Emma's primers)
(→Emma's primers)
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!Tm
!Tm
|-
|-
-
|*PhZ-UP
+
|*PhZ-Up
|5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|65.6
|65.6
|-
|-
-
|*PhZ-LOW
+
|*PhZ-Low
|5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
|5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
|64.8
|64.8
|-
|-
-
|*BbpPhZ_Up
+
|*Bbp_PhZ_Up
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|63.6
|63.6
|-
|-
-
|*BbsPhZ_Low
+
|*Bbs_PhZ_Low
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|64.2
|64.2
|-
|-
-
|BbpGene
+
|Bbp_Gene
|5' gaa ttc gcg gcc gct tct ag 3'
|5' gaa ttc gcg gcc gct tct ag 3'
|65.7
|65.7
Line 31: Line 31:
|65.3
|65.3
|-
|-
-
|*METHYL1
+
|*Methyl_1
|5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
|5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
|66.3
|66.3
|-
|-
-
|*METHYL2
+
|*Methyl_2
|5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
|5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
|66.2
|66.2
|-
|-
-
|*BbpMethyl1
+
|*Bbp_Methyl_1
|5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
|5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
|63.9
|63.9
|-
|-
-
|*BbsMethyl2
+
|*Bbs_Methyl_2
|5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
|5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
|66.9
|66.9
|-
|-
-
|PhzMfor1
+
|Phz_M_for_1
|5' atg aat aat tcg aat ctt gct gct 3'
|5' atg aat aat tcg aat ctt gct gct 3'
|65.4
|65.4
|-
|-
-
|PhzMrev1
+
|Phz_M_rev_1
|5' tca ggc cct ggc agc gac 3'
|5' tca ggc cct ggc agc gac 3'
|60.3
|60.3
|-
|-
-
|BbpPhzMfor1
+
|Bbp_Phz_M_for_1
|5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
|5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
|64.5
|64.5
|-
|-
-
|BbsPhzMrev1
+
|Bbs_Phz_M_rev_1
|5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
|5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
|60.3
|60.3
|-
|-
-
|*OXY1
+
|*Oxy_1
|5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
|5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
|60.2
|60.2
|-
|-
-
|*OXY2
+
|*Oxy_2
|5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
|5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
|59.2
|59.2
|-
|-
-
|*BbpOxy1
+
|*Bbp_Oxy_1
|5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
|5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
|65.1
|65.1
|-
|-
-
|*BbsOxy2
+
|*Bbs_Oxy_2
|5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
|5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
|66.9
|66.9
|-
|-
-
|PhzSfor1
+
|Phz_S_for_1
|5' atg agc gaa ccc atc gat at 3'
|5' atg agc gaa ccc atc gat at 3'
|71.2
|71.2
|-
|-
-
|PhzSrev1
+
|Phz_S_rev_1
|5' cta gcg tgg ccg ttc cac ctg 3'
|5' cta gcg tgg ccg ttc cac ctg 3'
|60.2
|60.2
|-
|-
-
|BbpPhzSfor1
+
|Bbp_Phz_S_for_1
|5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
|5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
|70.9
|70.9
|-
|-
-
|BbsPhzSrev1
+
|Bbs_Phz_S_rev_1
|5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
|5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
|63.3
|63.3
|-
|-
-
|PhzH_up
+
|Phz_H_up
|5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
|5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
|69.9
|69.9
|-
|-
-
|PhzH_low
+
|Phz_H_low
|5' GCC ACG CCA AGC TTC ACG CTC A 3'
|5' GCC ACG CCA AGC TTC ACG CTC A 3'
|70.6
|70.6
|-
|-
-
|BbpPhZ_Up
+
|Bbp_Phz_Up
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|63.3
|63.3
|-
|-
-
|BbsPhZ-Low
+
|Bbs_Phz_Low
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|57.7
|57.7
|}
|}

Revision as of 13:24, 24 July 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
*PhZ-Up 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 65.6
*PhZ-Low 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3' 64.8
*Bbp_PhZ_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.6
*Bbs_PhZ_Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 66.3
*Methyl_2 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.2
*Bbp_Methyl_1 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 63.9
*Bbs_Methyl_2 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.9
Phz_M_for_1 5' atg aat aat tcg aat ctt gct gct 3' 65.4
Phz_M_rev_1 5' tca ggc cct ggc agc gac 3' 60.3
Bbp_Phz_M_for_1 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3' 64.5
Bbs_Phz_M_rev_1 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3' 60.3
*Oxy_1 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 60.2
*Oxy_2 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 59.2
*Bbp_Oxy_1 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 65.1
*Bbs_Oxy_2 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 66.9
Phz_S_for_1 5' atg agc gaa ccc atc gat at 3' 71.2
Phz_S_rev_1 5' cta gcg tgg ccg ttc cac ctg 3' 60.2
Bbp_Phz_S_for_1 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3' 70.9
Bbs_Phz_S_rev_1 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3' 63.3
Phz_H_up 5' CGC ACG GAT CCT TTC AGA ATG TTC 3' 69.9
Phz_H_low 5' GCC ACG CCA AGC TTC ACG CTC A 3' 70.6
Bbp_Phz_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.3
Bbs_Phz_Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 57.7
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