Glasgow/Wetlab/Orders

From 2007.igem.org

< Glasgow | Wetlab(Difference between revisions)
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>])
 
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[[Glasgow|Glasgow Main Page]]
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{| valign=top cellpadding=3
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|-
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!align=center|[[Image:Uog.jpg]] ||    ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]]|| [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]]
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|}
----
----
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== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]==
 +
Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.
 +
----
 +
 +
== <font face=georgia color=#3366CC size=4>Primers</font> ==
 +
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
!Name
 +
!Sequence
 +
!Tm (°C)
 +
|-
 +
|DmpR_SDM_PstI_1_for
 +
|5´ CTGGAGCAACAGCATGCGCTGTTC 3´
 +
|65.6
 +
|-
 +
|DmpR_SDM_PstI_1_rev
 +
|5´ GCTCCAGTTTTCAGCGATGGCC 3´
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|64.8
 +
|-
 +
|DmpR_SDM_PstI_2_for
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|5´ CTGGAGGAAGTGCTCAACCAGCAG 3´
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|66.3
 +
|-
 +
|DmpR_SDM_PstI_2_rev
 +
|5´ CCTCCAGCGCTTCCACCAG 3´
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|66.2
 +
|-
 +
|DmpR_Start_Prefix_1_for
 +
|5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´
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|60.2
 +
|-
 +
|DmpR_End_Suffix_1_rev
 +
|5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´
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|59.2
 +
|-
 +
|PhzM_SDM_PstI_1_for
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|5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´
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|69.9
 +
|-
 +
|PhzM_SDM_PstI_1_rev
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|5´ CCTCCAGGCCGAGCCCAG 3´
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|70.6
 +
|-
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|PhzS_SDM_PstI_1_for
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|5´ CTGGAGTTCGCCGCGATGG 3´
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|65.7
 +
|-
 +
|PhzS_SDM_PstI_1_rev
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|5´ ACTCCAGATGATCCTGCTCGCC 3´
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|65.3
 +
|-
 +
|PhzS_SDM_PstI_2_for
 +
|5´ GTACTGGAGGATCAACTGGTTGCG 3´
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|63.6
 +
|-
 +
|PhzS_SDM_PstI_2_rev
 +
|5´ GTACTGGAGGATCAACTGGTTGCG 3´
 +
|63.9
 +
|-
 +
|PhzC_SDM_PstI_1_for
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|5´ CTGGAGCGCTTCGCAGCG 3´
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|65.1
 +
|-
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|PhzC_SDM_PstI_1_rev
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|5´ CCTCCAGCAACCCGAATGG 3´
 +
|64.2
 +
|-
 +
|PhzF_SDM_PstI_1_for
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|5´ GCACGCCACCACCTTGCC 3´
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|66.9
 +
|-
 +
|PhzF_SDM_PstI_1_rev
 +
|5´ GGCGTGCAGCATGCAGCAGC 3´
 +
|66.9
 +
|-
 +
|PhzB_SDM_EcoRI_1_for
 +
|5´ GAACTCGCGGTTGCGCTTGATCTTG 3´
 +
|65.4
 +
|-
 +
|PhzB_SDM_EcoRI_1_rev
 +
|5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´
 +
|64.5
 +
|-
 +
|PhzE_SDM_EcoRI_1_for
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|5´ GAACTCCATGACCTCGGC 3´
 +
|60.3
 +
|-
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|PhzE_SDM_EcoRI_1_rev
 +
|5´ GAGTTCCTCGACAACCGC 3´
 +
|60.3
 +
|-
 +
|PhzE_SDM_PstI_1_for
 +
|5´ CTGGAGGGCGCGCGCAGC 3´
 +
|71.2
 +
|-
 +
|PhzE_SDM_PstI_1_rev
 +
|5´ GCCCTCCAGGACCGCATCGAGATC 3´
 +
|70.9
 +
|-
 +
|XylR_SDM_PstI_1_for
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|5´ GAGCTCCAGACCCAGGTTG 3´
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|63.3
 +
|-
 +
|XylR_SDM_PstI_1_rev
 +
|5´ GTCTGGAGCTCGTAGCGCTC 3´
 +
|63.3
 +
|-
 +
|XylR_Prefix_1
 +
|5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´
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|57.7
 +
|-
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|XylR_Suffix_1
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|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´
 +
|59.8
 +
|-
 +
|Pu_Prefix_1(After XylR)
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|5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´
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|65.0
 +
|-
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|Pu_Suffix_1(After XylR)
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|5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´
 +
|65.1
 +
|-
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|Pu_Prefix_2(Emma´s sequence)
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|5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´
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|66.0
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|-
 +
|Pu_Suffix_2(Emma´s sequence)
 +
|5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´
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|66.4
 +
|-
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|DntR_Prefix_1
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|5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´
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|61.2
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|-
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|DntR_Suffix_1
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|5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´
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|55.2
 +
|-
 +
|DntR_Suffix_2
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|5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´
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|55.3
 +
|-
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|Pr_Prefix_1
 +
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´
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|58.8
 +
|-
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|Pr_Suffix_1
 +
|5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´
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|57.3
 +
|-
 +
|p1010_for
 +
|5´ GCTGTGTATAAGGGAGCCTGA 3´
 +
|59.4
 +
|-
 +
|p1010_rev
 +
|5´ TGACAGTTGACAGCGACAGC 3´
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|61.3
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|-
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|Bbp_XylR_for_2
 +
|5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´
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|55.2
 +
|-
 +
|Bbs_XylR_rev_2
 +
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´
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|61.8
 +
|-
 +
|Bbp_Pr_for_2
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|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´
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|60.8
 +
|-
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|Bbs_Pr_rev_2
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|5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´
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|61.3
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|-
 +
|Bbp_Pu_for_2
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|5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´
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|64.3
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|-
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|Bbs_Pu_rev_2
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|5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´
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|69.9
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|-
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|Bbp_PhzD_for
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|5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´
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|61.0
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|-
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|Bbs_PhzD_rev
 +
|5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´
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|61.4
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|-
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|Bbp_PhzA_for
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|5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´
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|63.1
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|-
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|Bbs_PhzG_rev
 +
|5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´
 +
|61.9
 +
|}
 +
----
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 +
== <font face=georgia color=#3366CC size=4>[[User:EmmaTravis|Emma]]'s Primers</font> ==
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{| border="1" cellspacing="0" cellpadding="5" align="center"
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!Name
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!Sequence
 +
!Tm (°C)
 +
|-
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|* PhzUp
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|5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
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|65.6
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|-
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|* PhzLow
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|5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
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|64.8
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|-
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|*Bbp_PhzUp
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|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
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|63.6
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|-
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|*Bbs_PhzLow
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|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
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|64.2
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|-
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|Bbp_Gene
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|5´ GAATTCGCGGCCGCTTCTAG 3´
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|65.7
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|-
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|Bbs
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|5´ CTGCAGCGGCCGCTACTAGTA 3´
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|65.3
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|-
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|*Methyl_1
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|5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
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|66.3
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|-
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|*Methyl_2
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|5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
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|66.2
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|-
 +
|*Bbp_Methyl_1
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|5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
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|63.9
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|-
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|*Bbs_Methyl_2
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|5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
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|66.9
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|-
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|PhzM_for_1
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|5´ atgaataattcgaatcttgctgct 3´
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|65.4
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|-
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|PhzM_rev_1
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|5´ tcaggccctggcagcgac 3´
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|60.3
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|-
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|Bbp_PhzM_for_1
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|5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´
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|64.5
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|-
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|Bbs_PhzM_rev_1
 +
|5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´
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|60.3
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|-
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|*Oxy_1
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|5´TCGAACACTCTAGAAAAGGAAGCACC  3´
 +
|60.2
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|-
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|*Oxy_2
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|5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´
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|59.2
 +
|-
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|*Bbp_Oxy_1
 +
|5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´
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|65.1
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|-
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|*Bbs_Oxy_2
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|5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´
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|66.9
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|-
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|PhzS_for_1
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|5´ atgagcgaacccatcgatat 3´
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|71.2
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|-
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|PhzS_rev_1
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|5´ ctagcgtggccgttccacctg 3´
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|60.2
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|-
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|Bbp_PhzS_for_1
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|5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´
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|70.9
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|-
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|Bbs_PhzS_rev_1
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|5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´
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|63.3
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|-
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|PhzH_up
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|5´ CGCACGGATCCTTTCAGAATGTTC 3´
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|69.9
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|-
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|PhzH_low
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|5´ GCCACGCCAAGCTTCACGCTCA 3´
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|70.6
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|-
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|Bbp_PhzUp
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|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
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|63.3
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|-
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|Bbs_PhzLow
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|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|57.7
 +
|}
 +
----
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{| valign=top cellpadding=3
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|-
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!align=center|[[Image:Uog.jpg]] ||  ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]] || [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]]
 +
|}

Latest revision as of 11:28, 26 October 2007

Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log		 Back To
Glasgow's
Project Page

Glasgow Parts Page

Visit the Glasgow Parts Page to see our constructs.

Primers

Name Sequence Tm (°C)
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
PhzM_SDM_PstI_1_for 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ 69.9
PhzM_SDM_PstI_1_rev 5´ CCTCCAGGCCGAGCCCAG 3´ 70.6
PhzS_SDM_PstI_1_for 5´ CTGGAGTTCGCCGCGATGG 3´ 65.7
PhzS_SDM_PstI_1_rev 5´ ACTCCAGATGATCCTGCTCGCC 3´ 65.3
PhzS_SDM_PstI_2_for 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.6
PhzS_SDM_PstI_2_rev 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.9
PhzC_SDM_PstI_1_for 5´ CTGGAGCGCTTCGCAGCG 3´ 65.1
PhzC_SDM_PstI_1_rev 5´ CCTCCAGCAACCCGAATGG 3´ 64.2
PhzF_SDM_PstI_1_for 5´ GCACGCCACCACCTTGCC 3´ 66.9
PhzF_SDM_PstI_1_rev 5´ GGCGTGCAGCATGCAGCAGC 3´ 66.9
PhzB_SDM_EcoRI_1_for 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ 65.4
PhzB_SDM_EcoRI_1_rev 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ 64.5
PhzE_SDM_EcoRI_1_for 5´ GAACTCCATGACCTCGGC 3´ 60.3
PhzE_SDM_EcoRI_1_rev 5´ GAGTTCCTCGACAACCGC 3´ 60.3
PhzE_SDM_PstI_1_for 5´ CTGGAGGGCGCGCGCAGC 3´ 71.2
PhzE_SDM_PstI_1_rev 5´ GCCCTCCAGGACCGCATCGAGATC 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
Bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
Bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
Bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
Bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
Bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
Bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
Bbp_PhzD_for 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ 61.0
Bbs_PhzD_rev 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ 61.4
Bbp_PhzA_for 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ 63.1
Bbs_PhzG_rev 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ 61.9

Emma's Primers

Name Sequence Tm (°C)
* PhzUp 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 65.6
* PhzLow 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.8
*Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.6
*Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.2
Bbp_Gene 5´ GAATTCGCGGCCGCTTCTAG 3´ 65.7
Bbs 5´ CTGCAGCGGCCGCTACTAGTA 3´ 65.3
*Methyl_1 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 66.3
*Methyl_2 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.2
*Bbp_Methyl_1 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 63.9
*Bbs_Methyl_2 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.9
PhzM_for_1 5´ atgaataattcgaatcttgctgct 3´ 65.4
PhzM_rev_1 5´ tcaggccctggcagcgac 3´ 60.3
Bbp_PhzM_for_1 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ 64.5
Bbs_PhzM_rev_1 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ 60.3
*Oxy_1 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ 60.2
*Oxy_2 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 59.2
*Bbp_Oxy_1 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ 65.1
*Bbs_Oxy_2 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 66.9
PhzS_for_1 5´ atgagcgaacccatcgatat 3´ 71.2
PhzS_rev_1 5´ ctagcgtggccgttccacctg 3´ 60.2
Bbp_PhzS_for_1 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ 70.9
Bbs_PhzS_rev_1 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ 63.3
PhzH_up 5´ CGCACGGATCCTTTCAGAATGTTC 3´ 69.9
PhzH_low 5´ GCCACGCCAAGCTTCACGCTCA 3´ 70.6
Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.3
Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 57.7
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Main Page 		Back To
Glasgow's
Wetlab Log 		Back To
Glasgow's
Project Page
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Orders"