Glasgow/Wetlab/Orders

From 2007.igem.org

< Glasgow | Wetlab(Difference between revisions)
(→Emma's primers)
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>])
 
(31 intermediate revisions not shown)
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[[Glasgow|Glasgow Main Page]]
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{| valign=top cellpadding=3
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----
+
-
== [[User:Emmatravis|Emma]]'s primers ==
+
-
{| border="1" cellspacing="0" cellpadding="5" align="center"
+
-
!Name
+
-
!Sequence
+
-
!Tm
+
|-
|-
-
|* ***Up
+
!align=center|[[Image:Uog.jpg]] ||   ||   [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]]|| [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]]
-
|5´ *** 3´
+
-
|65.6
+
-
|-
+
-
|* ***Low
+
-
|5´ *** 3´
+
-
|64.8
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-
|-
+
-
|*Bbp_***Up
+
-
|5´ *** 3´
+
-
|63.6
+
-
|-
+
-
|*Bbs_***Low
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-
|5´ *** 3´
+
-
|64.2
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-
|-
+
-
|Bbp_Gene
+
-
|5´ gaattcgcggccgcttctag 3´
+
-
|65.7
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-
|-
+
-
|Bbs
+
-
|5´ ctgcagcggccgctactagta 3´
+
-
|65.3
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-
|-
+
-
|*Methyl_1
+
-
|5´ *** 3´
+
-
|66.3
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-
|-
+
-
|*Methyl_2
+
-
|5´ *** 3´
+
-
|66.2
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-
|-
+
-
|*Bbp_Methyl_1
+
-
|5´ *** 3´
+
-
|63.9
+
-
|-
+
-
|*Bbs_Methyl_2
+
-
|5´ *** 3´
+
-
|66.9
+
-
|-
+
-
|***M_for_1
+
-
|5´ *** 3´
+
-
|65.4
+
-
|-
+
-
|***M_rev_1
+
-
|5´ *** 3´
+
-
|60.3
+
-
|-
+
-
|Bbp_***M_for_1
+
-
|5´ *** 3´
+
-
|64.5
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-
|-
+
-
|Bbs_***M_rev_1
+
-
|5´ *** 3´
+
-
|60.3
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-
|-
+
-
|*Oxy_1
+
-
|5´ *** 3´
+
-
|60.2
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-
|-
+
-
|*Oxy_2
+
-
|5´ *** 3´
+
-
|59.2
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-
|-
+
-
|*Bbp_Oxy_1
+
-
|5´ *** 3´
+
-
|65.1
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-
|-
+
-
|*Bbs_Oxy_2
+
-
|5´ *** 3´
+
-
|66.9
+
-
|-
+
-
|***S_for_1
+
-
|5´ *** 3´
+
-
|71.2
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-
|-
+
-
|***S_rev_1
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-
|5´ *** 3´
+
-
|60.2
+
-
|-
+
-
|Bbp_***S_for_1
+
-
|5´ *** 3´
+
-
|70.9
+
-
|-
+
-
|Bbs_***S_rev_1
+
-
|5´ *** 3´
+
-
|63.3
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-
|-
+
-
|***H_up
+
-
|5´ *** 3´
+
-
|69.9
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-
|-
+
-
|***H_low
+
-
|5´ *** 3´
+
-
|70.6
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-
|-
+
-
|Bbp_***Up
+
-
|5´ *** 3´
+
-
|63.3
+
-
|-
+
-
|Bbs_***Low
+
-
|5´ *** 3´
+
-
|57.7
+
|}
|}
 +
----
 +
== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]==
 +
Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.
 +
----
-
== Designed primers ==
+
== <font face=georgia color=#3366CC size=4>Primers</font> ==
{| border="1" cellspacing="0" cellpadding="5" align="center"
{| border="1" cellspacing="0" cellpadding="5" align="center"
!Name
!Name
!Sequence
!Sequence
-
!Tm
+
!Tm (°C)
|-
|-
|DmpR_SDM_PstI_1_for
|DmpR_SDM_PstI_1_for
-
|5´CTGGAGCAACAGCATGCGCTGTTC3´
+
|5´ CTGGAGCAACAGCATGCGCTGTTC 3´
|65.6
|65.6
|-
|-
|DmpR_SDM_PstI_1_rev
|DmpR_SDM_PstI_1_rev
-
|5´GCTCCAGTTTTCAGCGATGGCC3´
+
|5´ GCTCCAGTTTTCAGCGATGGCC 3´
|64.8
|64.8
|-
|-
|DmpR_SDM_PstI_2_for
|DmpR_SDM_PstI_2_for
-
|5´CTGGAGGAAGTGCTCAACCAGCAG3´
+
|5´ CTGGAGGAAGTGCTCAACCAGCAG 3´
|66.3
|66.3
|-
|-
|DmpR_SDM_PstI_2_rev
|DmpR_SDM_PstI_2_rev
-
|5´CCTCCAGCGCTTCCACCAG3´
+
|5´ CCTCCAGCGCTTCCACCAG 3´
|66.2
|66.2
|-
|-
|DmpR_Start_Prefix_1_for
|DmpR_Start_Prefix_1_for
-
|5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´
|60.2
|60.2
|-
|-
|DmpR_End_Suffix_1_rev
|DmpR_End_Suffix_1_rev
-
|5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´
|59.2
|59.2
|-
|-
-
|***M_SDM_PstI_1_for
+
|PhzM_SDM_PstI_1_for
-
|5´***3´
+
|5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´
|69.9
|69.9
|-
|-
-
|***M_SDM_PstI_1_rev
+
|PhzM_SDM_PstI_1_rev
-
|5´***3´
+
|5´ CCTCCAGGCCGAGCCCAG 3´
|70.6
|70.6
|-
|-
-
|***S_SDM_PstI_1_for
+
|PhzS_SDM_PstI_1_for
-
|5´***3´
+
|5´ CTGGAGTTCGCCGCGATGG 3´
|65.7
|65.7
|-
|-
-
|***S_SDM_PstI_1_rev
+
|PhzS_SDM_PstI_1_rev
-
|5´***3´
+
|5´ ACTCCAGATGATCCTGCTCGCC 3´
|65.3
|65.3
|-
|-
-
|***S_SDM_PstI_2_for
+
|PhzS_SDM_PstI_2_for
-
|5´***3´
+
|5´ GTACTGGAGGATCAACTGGTTGCG 3´
|63.6
|63.6
|-
|-
-
|***S_SDM_PstI_2_rev
+
|PhzS_SDM_PstI_2_rev
-
|5´***3´
+
|5´ GTACTGGAGGATCAACTGGTTGCG 3´
|63.9
|63.9
|-
|-
-
|***C_SDM_PstI_1_for
+
|PhzC_SDM_PstI_1_for
-
|5´***3´
+
|5´ CTGGAGCGCTTCGCAGCG 3´
|65.1
|65.1
|-
|-
-
|***C_SDM_PstI_1_rev
+
|PhzC_SDM_PstI_1_rev
-
|5´***3´
+
|5´ CCTCCAGCAACCCGAATGG 3´
|64.2
|64.2
|-
|-
-
|***F_SDM_PstI_1_for
+
|PhzF_SDM_PstI_1_for
-
|5´***3´
+
|5´ GCACGCCACCACCTTGCC 3´
|66.9
|66.9
|-
|-
-
|***F_SDM_PstI_1_rev
+
|PhzF_SDM_PstI_1_rev
-
|5´***3´
+
|5´ GGCGTGCAGCATGCAGCAGC 3´
|66.9
|66.9
|-
|-
-
|***B_SDM_EcoRI_1_for
+
|PhzB_SDM_EcoRI_1_for
-
|5´***3´
+
|5´ GAACTCGCGGTTGCGCTTGATCTTG 3´
|65.4
|65.4
|-
|-
-
|***B_SDM_EcoRI_1_rev
+
|PhzB_SDM_EcoRI_1_rev
-
|5´***3´
+
|5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´
|64.5
|64.5
|-
|-
-
|***E_SDM_EcoRI_1_for
+
|PhzE_SDM_EcoRI_1_for
-
|5´***3´
+
|5´ GAACTCCATGACCTCGGC 3´
|60.3
|60.3
|-
|-
-
|***E_SDM_EcoRI_1_rev
+
|PhzE_SDM_EcoRI_1_rev
-
|5´***3´
+
|5´ GAGTTCCTCGACAACCGC 3´
|60.3
|60.3
|-
|-
-
|***E_SDM_PstI_1_for
+
|PhzE_SDM_PstI_1_for
-
|5´***3´
+
|5´ CTGGAGGGCGCGCGCAGC 3´
|71.2
|71.2
|-
|-
-
|***E_SDM_PstI_1_rev
+
|PhzE_SDM_PstI_1_rev
-
|5´***3´
+
|5´ GCCCTCCAGGACCGCATCGAGATC 3´
|70.9
|70.9
|-
|-
|XylR_SDM_PstI_1_for
|XylR_SDM_PstI_1_for
-
|5´GAGCTCCAGACCCAGGTTG3´
+
|5´ GAGCTCCAGACCCAGGTTG 3´
|63.3
|63.3
|-
|-
|XylR_SDM_PstI_1_rev
|XylR_SDM_PstI_1_rev
-
|5´GTCTGGAGCTCGTAGCGCTC3´
+
|5´ GTCTGGAGCTCGTAGCGCTC 3´
|63.3
|63.3
|-
|-
|XylR_Prefix_1
|XylR_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´
|57.7
|57.7
|-
|-
|XylR_Suffix_1
|XylR_Suffix_1
-
|5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´
|59.8
|59.8
|-
|-
|Pu_Prefix_1(After XylR)
|Pu_Prefix_1(After XylR)
-
|5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´
|65.0
|65.0
|-
|-
|Pu_Suffix_1(After XylR)
|Pu_Suffix_1(After XylR)
-
|5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´
+
|5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´
|65.1
|65.1
|-
|-
|Pu_Prefix_2(Emma´s sequence)
|Pu_Prefix_2(Emma´s sequence)
-
|5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´
|66.0
|66.0
|-
|-
|Pu_Suffix_2(Emma´s sequence)
|Pu_Suffix_2(Emma´s sequence)
-
|5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´
|66.4
|66.4
|-
|-
|DntR_Prefix_1
|DntR_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´
|61.2
|61.2
|-
|-
|DntR_Suffix_1
|DntR_Suffix_1
-
|5´TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT3´
+
|5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´
|55.2
|55.2
|-
|-
|DntR_Suffix_2
|DntR_Suffix_2
-
|5´CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG3´
+
|5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´
|55.3
|55.3
|-
|-
|Pr_Prefix_1
|Pr_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´
|58.8
|58.8
|-
|-
|Pr_Suffix_1
|Pr_Suffix_1
-
|5´CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´
|57.3
|57.3
|-
|-
|p1010_for
|p1010_for
-
|5´GCTGTGTATAAGGGAGCCTGA3´
+
|5´ GCTGTGTATAAGGGAGCCTGA 3´
|59.4
|59.4
|-
|-
|p1010_rev
|p1010_rev
-
|5´TGACAGTTGACAGCGACAGC3´
+
|5´ TGACAGTTGACAGCGACAGC 3´
|61.3
|61.3
|-
|-
-
|bbp_XylR_for_2
+
|Bbp_XylR_for_2
-
|5´GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC3´
+
|5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´
|55.2
|55.2
|-
|-
-
|bbs_XylR_rev_2
+
|Bbs_XylR_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´
|61.8
|61.8
|-
|-
-
|bbp_Pr_for_2
+
|Bbp_Pr_for_2
-
|5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´
|60.8
|60.8
|-
|-
-
|bbs_Pr_rev_2
+
|Bbs_Pr_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´
|61.3
|61.3
|-
|-
-
|bbp_Pu_for_2
+
|Bbp_Pu_for_2
-
|5´GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´
|64.3
|64.3
|-
|-
-
|bbs_Pu_rev_2
+
|Bbs_Pu_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´
|69.9
|69.9
|-
|-
-
|bbp_***D_for
+
|Bbp_PhzD_for
-
|5´***3´
+
|5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´
|61.0
|61.0
|-
|-
-
|bbs_***D_for
+
|Bbs_PhzD_rev
-
|5´***3´
+
|5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´
|61.4
|61.4
|-
|-
-
|bbp_***A_for
+
|Bbp_PhzA_for
-
|5´***3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´
|63.1
|63.1
|-
|-
-
|bbs_***G_rev
+
|Bbs_PhzG_rev
-
|5´***3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´
|61.9
|61.9
 +
|}
 +
----
 +
 +
== <font face=georgia color=#3366CC size=4>[[User:EmmaTravis|Emma]]'s Primers</font> ==
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
!Name
 +
!Sequence
 +
!Tm (°C)
 +
|-
 +
|* PhzUp
 +
|5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|65.6
 +
|-
 +
|* PhzLow
 +
|5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|64.8
 +
|-
 +
|*Bbp_PhzUp
 +
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|63.6
 +
|-
 +
|*Bbs_PhzLow
 +
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|64.2
 +
|-
 +
|Bbp_Gene
 +
|5´ GAATTCGCGGCCGCTTCTAG 3´
 +
|65.7
 +
|-
 +
|Bbs
 +
|5´ CTGCAGCGGCCGCTACTAGTA 3´
 +
|65.3
 +
|-
 +
|*Methyl_1
 +
|5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
 +
|66.3
 +
|-
 +
|*Methyl_2
 +
|5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
 +
|66.2
 +
|-
 +
|*Bbp_Methyl_1
 +
|5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
 +
|63.9
 +
|-
 +
|*Bbs_Methyl_2
 +
|5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
 +
|66.9
 +
|-
 +
|PhzM_for_1
 +
|5´ atgaataattcgaatcttgctgct 3´
 +
|65.4
 +
|-
 +
|PhzM_rev_1
 +
|5´ tcaggccctggcagcgac 3´
 +
|60.3
 +
|-
 +
|Bbp_PhzM_for_1
 +
|5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´
 +
|64.5
 +
|-
 +
|Bbs_PhzM_rev_1
 +
|5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´
 +
|60.3
 +
|-
 +
|*Oxy_1
 +
|5´TCGAACACTCTAGAAAAGGAAGCACC  3´
 +
|60.2
 +
|-
 +
|*Oxy_2
 +
|5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´
 +
|59.2
 +
|-
 +
|*Bbp_Oxy_1
 +
|5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´
 +
|65.1
 +
|-
 +
|*Bbs_Oxy_2
 +
|5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´
 +
|66.9
 +
|-
 +
|PhzS_for_1
 +
|5´ atgagcgaacccatcgatat 3´
 +
|71.2
 +
|-
 +
|PhzS_rev_1
 +
|5´ ctagcgtggccgttccacctg 3´
 +
|60.2
 +
|-
 +
|Bbp_PhzS_for_1
 +
|5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´
 +
|70.9
 +
|-
 +
|Bbs_PhzS_rev_1
 +
|5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´
 +
|63.3
 +
|-
 +
|PhzH_up
 +
|5´ CGCACGGATCCTTTCAGAATGTTC 3´
 +
|69.9
 +
|-
 +
|PhzH_low
 +
|5´ GCCACGCCAAGCTTCACGCTCA 3´
 +
|70.6
 +
|-
 +
|Bbp_PhzUp
 +
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|63.3
 +
|-
 +
|Bbs_PhzLow
 +
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|57.7
 +
|}
 +
----
 +
{| valign=top cellpadding=3
 +
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Latest revision as of 11:28, 26 October 2007

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Primers

Name Sequence Tm (°C)
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
PhzM_SDM_PstI_1_for 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ 69.9
PhzM_SDM_PstI_1_rev 5´ CCTCCAGGCCGAGCCCAG 3´ 70.6
PhzS_SDM_PstI_1_for 5´ CTGGAGTTCGCCGCGATGG 3´ 65.7
PhzS_SDM_PstI_1_rev 5´ ACTCCAGATGATCCTGCTCGCC 3´ 65.3
PhzS_SDM_PstI_2_for 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.6
PhzS_SDM_PstI_2_rev 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.9
PhzC_SDM_PstI_1_for 5´ CTGGAGCGCTTCGCAGCG 3´ 65.1
PhzC_SDM_PstI_1_rev 5´ CCTCCAGCAACCCGAATGG 3´ 64.2
PhzF_SDM_PstI_1_for 5´ GCACGCCACCACCTTGCC 3´ 66.9
PhzF_SDM_PstI_1_rev 5´ GGCGTGCAGCATGCAGCAGC 3´ 66.9
PhzB_SDM_EcoRI_1_for 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ 65.4
PhzB_SDM_EcoRI_1_rev 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ 64.5
PhzE_SDM_EcoRI_1_for 5´ GAACTCCATGACCTCGGC 3´ 60.3
PhzE_SDM_EcoRI_1_rev 5´ GAGTTCCTCGACAACCGC 3´ 60.3
PhzE_SDM_PstI_1_for 5´ CTGGAGGGCGCGCGCAGC 3´ 71.2
PhzE_SDM_PstI_1_rev 5´ GCCCTCCAGGACCGCATCGAGATC 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
Bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
Bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
Bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
Bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
Bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
Bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
Bbp_PhzD_for 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ 61.0
Bbs_PhzD_rev 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ 61.4
Bbp_PhzA_for 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ 63.1
Bbs_PhzG_rev 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ 61.9

Emma's Primers

Name Sequence Tm (°C)
* PhzUp 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 65.6
* PhzLow 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.8
*Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.6
*Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.2
Bbp_Gene 5´ GAATTCGCGGCCGCTTCTAG 3´ 65.7
Bbs 5´ CTGCAGCGGCCGCTACTAGTA 3´ 65.3
*Methyl_1 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 66.3
*Methyl_2 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.2
*Bbp_Methyl_1 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 63.9
*Bbs_Methyl_2 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.9
PhzM_for_1 5´ atgaataattcgaatcttgctgct 3´ 65.4
PhzM_rev_1 5´ tcaggccctggcagcgac 3´ 60.3
Bbp_PhzM_for_1 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ 64.5
Bbs_PhzM_rev_1 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ 60.3
*Oxy_1 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ 60.2
*Oxy_2 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 59.2
*Bbp_Oxy_1 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ 65.1
*Bbs_Oxy_2 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 66.9
PhzS_for_1 5´ atgagcgaacccatcgatat 3´ 71.2
PhzS_rev_1 5´ ctagcgtggccgttccacctg 3´ 60.2
Bbp_PhzS_for_1 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ 70.9
Bbs_PhzS_rev_1 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ 63.3
PhzH_up 5´ CGCACGGATCCTTTCAGAATGTTC 3´ 69.9
PhzH_low 5´ GCCACGCCAAGCTTCACGCTCA 3´ 70.6
Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.3
Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 57.7
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