Glasgow/Wetlab/Orders

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< Glasgow | Wetlab(Difference between revisions)
(→Designed primers)
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>])
 
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== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow's Sandbox] ==
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== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]==
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Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.
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== Designed primers ==
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== <font face=georgia color=#3366CC size=4>Primers</font> ==
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== [[User:EmmaTravis|Emma]]'s primers ==
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== <font face=georgia color=#3366CC size=4>[[User:EmmaTravis|Emma]]'s Primers</font> ==
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!Name
!Name
!Sequence
!Sequence
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!Tm
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!Tm (°C)
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|* PhzUp
|* PhzUp
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!align=center|[[Image:Uog.jpg]] ||  ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]]
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!align=center|[[Image:Uog.jpg]] ||  ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]] || [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]]
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Latest revision as of 11:28, 26 October 2007

Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log		 Back To
Glasgow's
Project Page

Glasgow Parts Page

Visit the Glasgow Parts Page to see our constructs.

Primers

Name Sequence Tm (°C)
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
PhzM_SDM_PstI_1_for 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ 69.9
PhzM_SDM_PstI_1_rev 5´ CCTCCAGGCCGAGCCCAG 3´ 70.6
PhzS_SDM_PstI_1_for 5´ CTGGAGTTCGCCGCGATGG 3´ 65.7
PhzS_SDM_PstI_1_rev 5´ ACTCCAGATGATCCTGCTCGCC 3´ 65.3
PhzS_SDM_PstI_2_for 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.6
PhzS_SDM_PstI_2_rev 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.9
PhzC_SDM_PstI_1_for 5´ CTGGAGCGCTTCGCAGCG 3´ 65.1
PhzC_SDM_PstI_1_rev 5´ CCTCCAGCAACCCGAATGG 3´ 64.2
PhzF_SDM_PstI_1_for 5´ GCACGCCACCACCTTGCC 3´ 66.9
PhzF_SDM_PstI_1_rev 5´ GGCGTGCAGCATGCAGCAGC 3´ 66.9
PhzB_SDM_EcoRI_1_for 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ 65.4
PhzB_SDM_EcoRI_1_rev 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ 64.5
PhzE_SDM_EcoRI_1_for 5´ GAACTCCATGACCTCGGC 3´ 60.3
PhzE_SDM_EcoRI_1_rev 5´ GAGTTCCTCGACAACCGC 3´ 60.3
PhzE_SDM_PstI_1_for 5´ CTGGAGGGCGCGCGCAGC 3´ 71.2
PhzE_SDM_PstI_1_rev 5´ GCCCTCCAGGACCGCATCGAGATC 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
Bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
Bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
Bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
Bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
Bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
Bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
Bbp_PhzD_for 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ 61.0
Bbs_PhzD_rev 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ 61.4
Bbp_PhzA_for 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ 63.1
Bbs_PhzG_rev 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ 61.9

Emma's Primers

Name Sequence Tm (°C)
* PhzUp 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 65.6
* PhzLow 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.8
*Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.6
*Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.2
Bbp_Gene 5´ GAATTCGCGGCCGCTTCTAG 3´ 65.7
Bbs 5´ CTGCAGCGGCCGCTACTAGTA 3´ 65.3
*Methyl_1 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 66.3
*Methyl_2 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.2
*Bbp_Methyl_1 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 63.9
*Bbs_Methyl_2 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.9
PhzM_for_1 5´ atgaataattcgaatcttgctgct 3´ 65.4
PhzM_rev_1 5´ tcaggccctggcagcgac 3´ 60.3
Bbp_PhzM_for_1 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ 64.5
Bbs_PhzM_rev_1 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ 60.3
*Oxy_1 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ 60.2
*Oxy_2 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 59.2
*Bbp_Oxy_1 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ 65.1
*Bbs_Oxy_2 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 66.9
PhzS_for_1 5´ atgagcgaacccatcgatat 3´ 71.2
PhzS_rev_1 5´ ctagcgtggccgttccacctg 3´ 60.2
Bbp_PhzS_for_1 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ 70.9
Bbs_PhzS_rev_1 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ 63.3
PhzH_up 5´ CGCACGGATCCTTTCAGAATGTTC 3´ 69.9
PhzH_low 5´ GCCACGCCAAGCTTCACGCTCA 3´ 70.6
Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.3
Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 57.7
Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log 		Back To
Glasgow's
Project Page
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Orders"