Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Designed primers)
Line 4: Line 4:
|}
|}
----
----
-
== [[User:EmmaTravis|Emma]]'s primers ==
+
== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow's Sandbox ==
-
{| border="1" cellspacing="0" cellpadding="5" align="center"
+
----
-
!Name
+
-
!Sequence
+
-
!Tm
+
-
|-
+
-
|* PhzUp
+
-
|5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
+
-
|65.6
+
-
|-
+
-
|* PhzLow
+
-
|5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
+
-
|64.8
+
-
|-
+
-
|*Bbp_PhzUp
+
-
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
+
-
|63.6
+
-
|-
+
-
|*Bbs_PhzLow
+
-
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
+
-
|64.2
+
-
|-
+
-
|Bbp_Gene
+
-
|5´ GAATTCGCGGCCGCTTCTAG 3´
+
-
|65.7
+
-
|-
+
-
|Bbs
+
-
|5´ CTGCAGCGGCCGCTACTAGTA 3´
+
-
|65.3
+
-
|-
+
-
|*Methyl_1
+
-
|5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
+
-
|66.3
+
-
|-
+
-
|*Methyl_2
+
-
|5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
+
-
|66.2
+
-
|-
+
-
|*Bbp_Methyl_1
+
-
|5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
+
-
|63.9
+
-
|-
+
-
|*Bbs_Methyl_2
+
-
|5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
+
-
|66.9
+
-
|-
+
-
|PhzM_for_1
+
-
|5´ atgaataattcgaatcttgctgct 3´
+
-
|65.4
+
-
|-
+
-
|PhzM_rev_1
+
-
|5´ tcaggccctggcagcgac 3´
+
-
|60.3
+
-
|-
+
-
|Bbp_PhzM_for_1
+
-
|5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´
+
-
|64.5
+
-
|-
+
-
|Bbs_PhzM_rev_1
+
-
|5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´
+
-
|60.3
+
-
|-
+
-
|*Oxy_1
+
-
|5´TCGAACACTCTAGAAAAGGAAGCACC  3´
+
-
|60.2
+
-
|-
+
-
|*Oxy_2
+
-
|5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´
+
-
|59.2
+
-
|-
+
-
|*Bbp_Oxy_1
+
-
|5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´
+
-
|65.1
+
-
|-
+
-
|*Bbs_Oxy_2
+
-
|5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´
+
-
|66.9
+
-
|-
+
-
|PhzS_for_1
+
-
|5´ atgagcgaacccatcgatat 3´
+
-
|71.2
+
-
|-
+
-
|PhzS_rev_1
+
-
|5´ ctagcgtggccgttccacctg 3´
+
-
|60.2
+
-
|-
+
-
|Bbp_PhzS_for_1
+
-
|5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´
+
-
|70.9
+
-
|-
+
-
|Bbs_PhzS_rev_1
+
-
|5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´
+
-
|63.3
+
-
|-
+
-
|PhzH_up
+
-
|5´ CGCACGGATCCTTTCAGAATGTTC 3´
+
-
|69.9
+
-
|-
+
-
|PhzH_low
+
-
|5´ GCCACGCCAAGCTTCACGCTCA 3´
+
-
|70.6
+
-
|-
+
-
|Bbp_PhzUp
+
-
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
+
-
|63.3
+
-
|-
+
-
|Bbs_PhzLow
+
-
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
+
-
|57.7
+
-
|}
+
== Designed primers ==
== Designed primers ==
Line 309: Line 201:
|5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´
|5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´
|61.9
|61.9
 +
|}
 +
----
 +
== [[User:EmmaTravis|Emma]]'s primers ==
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
!Name
 +
!Sequence
 +
!Tm
 +
|-
 +
|* PhzUp
 +
|5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|65.6
 +
|-
 +
|* PhzLow
 +
|5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|64.8
 +
|-
 +
|*Bbp_PhzUp
 +
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|63.6
 +
|-
 +
|*Bbs_PhzLow
 +
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|64.2
 +
|-
 +
|Bbp_Gene
 +
|5´ GAATTCGCGGCCGCTTCTAG 3´
 +
|65.7
 +
|-
 +
|Bbs
 +
|5´ CTGCAGCGGCCGCTACTAGTA 3´
 +
|65.3
 +
|-
 +
|*Methyl_1
 +
|5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
 +
|66.3
 +
|-
 +
|*Methyl_2
 +
|5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
 +
|66.2
 +
|-
 +
|*Bbp_Methyl_1
 +
|5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´
 +
|63.9
 +
|-
 +
|*Bbs_Methyl_2
 +
|5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´
 +
|66.9
 +
|-
 +
|PhzM_for_1
 +
|5´ atgaataattcgaatcttgctgct 3´
 +
|65.4
 +
|-
 +
|PhzM_rev_1
 +
|5´ tcaggccctggcagcgac 3´
 +
|60.3
 +
|-
 +
|Bbp_PhzM_for_1
 +
|5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´
 +
|64.5
 +
|-
 +
|Bbs_PhzM_rev_1
 +
|5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´
 +
|60.3
 +
|-
 +
|*Oxy_1
 +
|5´TCGAACACTCTAGAAAAGGAAGCACC  3´
 +
|60.2
 +
|-
 +
|*Oxy_2
 +
|5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´
 +
|59.2
 +
|-
 +
|*Bbp_Oxy_1
 +
|5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´
 +
|65.1
 +
|-
 +
|*Bbs_Oxy_2
 +
|5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´
 +
|66.9
 +
|-
 +
|PhzS_for_1
 +
|5´ atgagcgaacccatcgatat 3´
 +
|71.2
 +
|-
 +
|PhzS_rev_1
 +
|5´ ctagcgtggccgttccacctg 3´
 +
|60.2
 +
|-
 +
|Bbp_PhzS_for_1
 +
|5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´
 +
|70.9
 +
|-
 +
|Bbs_PhzS_rev_1
 +
|5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´
 +
|63.3
 +
|-
 +
|PhzH_up
 +
|5´ CGCACGGATCCTTTCAGAATGTTC 3´
 +
|69.9
 +
|-
 +
|PhzH_low
 +
|5´ GCCACGCCAAGCTTCACGCTCA 3´
 +
|70.6
 +
|-
 +
|Bbp_PhzUp
 +
|5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´
 +
|63.3
 +
|-
 +
|Bbs_PhzLow
 +
|5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´
 +
|57.7
|}
|}
----
----

Revision as of 14:27, 25 October 2007

Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log		 Back To
Glasgow's
Project Page

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow's Sandbox

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
PhzM_SDM_PstI_1_for 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ 69.9
PhzM_SDM_PstI_1_rev 5´ CCTCCAGGCCGAGCCCAG 3´ 70.6
PhzS_SDM_PstI_1_for 5´ CTGGAGTTCGCCGCGATGG 3´ 65.7
PhzS_SDM_PstI_1_rev 5´ ACTCCAGATGATCCTGCTCGCC 3´ 65.3
PhzS_SDM_PstI_2_for 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.6
PhzS_SDM_PstI_2_rev 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.9
PhzC_SDM_PstI_1_for 5´ CTGGAGCGCTTCGCAGCG 3´ 65.1
PhzC_SDM_PstI_1_rev 5´ CCTCCAGCAACCCGAATGG 3´ 64.2
PhzF_SDM_PstI_1_for 5´ GCACGCCACCACCTTGCC 3´ 66.9
PhzF_SDM_PstI_1_rev 5´ GGCGTGCAGCATGCAGCAGC 3´ 66.9
PhzB_SDM_EcoRI_1_for 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ 65.4
PhzB_SDM_EcoRI_1_rev 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ 64.5
PhzE_SDM_EcoRI_1_for 5´ GAACTCCATGACCTCGGC 3´ 60.3
PhzE_SDM_EcoRI_1_rev 5´ GAGTTCCTCGACAACCGC 3´ 60.3
PhzE_SDM_PstI_1_for 5´ CTGGAGGGCGCGCGCAGC 3´ 71.2
PhzE_SDM_PstI_1_rev 5´ GCCCTCCAGGACCGCATCGAGATC 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
Bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
Bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
Bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
Bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
Bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
Bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
Bbp_PhzD_for 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ 61.0
Bbs_PhzD_rev 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ 61.4
Bbp_PhzA_for 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ 63.1
Bbs_PhzG_rev 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ 61.9

Emma's primers

Name Sequence Tm
* PhzUp 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 65.6
* PhzLow 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.8
*Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.6
*Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.2
Bbp_Gene 5´ GAATTCGCGGCCGCTTCTAG 3´ 65.7
Bbs 5´ CTGCAGCGGCCGCTACTAGTA 3´ 65.3
*Methyl_1 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 66.3
*Methyl_2 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.2
*Bbp_Methyl_1 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 63.9
*Bbs_Methyl_2 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.9
PhzM_for_1 5´ atgaataattcgaatcttgctgct 3´ 65.4
PhzM_rev_1 5´ tcaggccctggcagcgac 3´ 60.3
Bbp_PhzM_for_1 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ 64.5
Bbs_PhzM_rev_1 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ 60.3
*Oxy_1 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ 60.2
*Oxy_2 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 59.2
*Bbp_Oxy_1 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ 65.1
*Bbs_Oxy_2 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 66.9
PhzS_for_1 5´ atgagcgaacccatcgatat 3´ 71.2
PhzS_rev_1 5´ ctagcgtggccgttccacctg 3´ 60.2
Bbp_PhzS_for_1 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ 70.9
Bbs_PhzS_rev_1 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ 63.3
PhzH_up 5´ CGCACGGATCCTTTCAGAATGTTC 3´ 69.9
PhzH_low 5´ GCCACGCCAAGCTTCACGCTCA 3´ 70.6
Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.3
Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 57.7
Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Orders"