Glasgow/Wetlab/Orders

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Emma's primers

Name Sequence Tm
*PhZ-Up 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 65.6
*PhZ-Low 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3' 64.8
*Bbp_PhZ_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.6
*Bbs_PhZ_Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 66.3
*Methyl_2 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.2
*Bbp_Methyl_1 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 63.9
*Bbs_Methyl_2 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.9
Phz_M_for_1 5' atg aat aat tcg aat ctt gct gct 3' 65.4
Phz_M_rev_1 5' tca ggc cct ggc agc gac 3' 60.3
Bbp_Phz_M_for_1 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3' 64.5
Bbs_Phz_M_rev_1 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3' 60.3
*Oxy_1 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 60.2
*Oxy_2 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 59.2
*Bbp_Oxy_1 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 65.1
*Bbs_Oxy_2 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 66.9
Phz_S_for_1 5' atg agc gaa ccc atc gat at 3' 71.2
Phz_S_rev_1 5' cta gcg tgg ccg ttc cac ctg 3' 60.2
Bbp_Phz_S_for_1 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3' 70.9
Bbs_Phz_S_rev_1 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3' 63.3
Phz_H_up 5' CGC ACG GAT CCT TTC AGA ATG TTC 3' 69.9
Phz_H_low 5' GCC ACG CCA AGC TTC ACG CTC A 3' 70.6
Bbp_Phz_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.3
Bbs_Phz_Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 57.7

Primers marked with * are taken from previous studies.