Glasgow/Wetlab/Orders

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Emma's primers

Name Sequence Tm
*(***)-Up 5' *** C 3' 65.6
*(***)-Low 5' *** 3' 64.8
*Bbp_(***)_Up 5' *** 3' 63.6
*Bbs_(***)_Low 5' *** 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' *** 3' 66.3
*Methyl_2 5' *** 3' 66.2
*Bbp_Methyl_1 5' *** 3' 63.9
*Bbs_Methyl_2 5' *** 3' 66.9
(***)_M_for_1 5' *** 3' 65.4
(***)_M_rev_1 5' *** 3' 60.3
Bbp_(***)_M_for_1 5' *** 3' 64.5
Bbs_(***)_M_rev_1 5' *** 3' 60.3
*Oxy_1 5' *** 3' 60.2
*Oxy_2 5' *** 3' 59.2
*Bbp_Oxy_1 5' *** 3' 65.1
*Bbs_Oxy_2 5' *** 3' 66.9
(***)_S_for_1 5' *** 3' 71.2
(***)_S_rev_1 5' *** 3' 60.2
Bbp_(***)_S_for_1 5' *** 3' 70.9
Bbs_(***)_S_rev_1 5' *** 3' 63.3
(***)_H_up 5' *** 3' 69.9
(***)_H_low 5' *** 3' 70.6
Bbp_(***)_Up 5' *** 3' 63.3
Bbs_(***)_Low 5' *** 3' 57.7

Designed primers