Glasgow/Wetlab/Orders

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Emma's primers

Name Sequence Tm
* ***Up 5´ *** 3´ 65.6
* ***Low 5´ *** 3´ 64.8
*Bbp_***Up 5´ *** 3´ 63.6
*Bbs_***Low 5´ *** 3´ 64.2
Bbp_Gene 5´ gaattcgcggccgcttctag 3´ 65.7
Bbs 5´ ctgcagcggccgctactagta 3´ 65.3
*Methyl_1 5´ *** 3´ 66.3
*Methyl_2 5´ *** 3´ 66.2
*Bbp_Methyl_1 5´ *** 3´ 63.9
*Bbs_Methyl_2 5´ *** 3´ 66.9
***M_for_1 5´ *** 3´ 65.4
***M_rev_1 5´ *** 3´ 60.3
Bbp_***M_for_1 5´ *** 3´ 64.5
Bbs_***M_rev_1 5´ *** 3´ 60.3
*Oxy_1 5´ *** 3´ 60.2
*Oxy_2 5´ *** 3´ 59.2
*Bbp_Oxy_1 5´ *** 3´ 65.1
*Bbs_Oxy_2 5´ *** 3´ 66.9
***S_for_1 5´ *** 3´ 71.2
***S_rev_1 5´ *** 3´ 60.2
Bbp_***S_for_1 5´ *** 3´ 70.9
Bbs_***S_rev_1 5´ *** 3´ 63.3
***H_up 5´ *** 3´ 69.9
***H_low 5´ *** 3´ 70.6
Bbp_***Up 5´ *** 3´ 63.3
Bbs_***Low 5´ *** 3´ 57.7

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
***M_SDM_PstI_1_for 5´ *** 3´ 69.9
***M_SDM_PstI_1_rev 5´ *** 3´ 70.6
***S_SDM_PstI_1_for 5´ *** 3´ 65.7
***S_SDM_PstI_1_rev 5´ *** 3´ 65.3
***S_SDM_PstI_2_for 5´ *** 3´ 63.6
***S_SDM_PstI_2_rev 5´ *** 3´ 63.9
***C_SDM_PstI_1_for 5´ *** 3´ 65.1
***C_SDM_PstI_1_rev 5´ *** 3´ 64.2
***F_SDM_PstI_1_for 5´ *** 3´ 66.9
***F_SDM_PstI_1_rev 5´ *** 3´ 66.9
***B_SDM_EcoRI_1_for 5´ *** 3´ 65.4
***B_SDM_EcoRI_1_rev 5´ *** 3´ 64.5
***E_SDM_EcoRI_1_for 5´ *** 3´ 60.3
***E_SDM_EcoRI_1_rev 5´ *** 3´ 60.3
***E_SDM_PstI_1_for 5´ *** 3´ 71.2
***E_SDM_PstI_1_rev 5´ *** 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
bbp_***D_for 5´ *** 3´ 61.0
bbs_***D_for 5´ *** 3´ 61.4
bbp_***A_for 5´ *** 3´ 63.1
bbs_***G_rev 5´ *** 3´ 61.9