Glasgow/Wetlab/Week10
From 2007.igem.org
(Difference between revisions)
(→Monday 3st September 2007) |
(→Monday 3st September 2007) |
||
| Line 47: | Line 47: | ||
|(*d→g*) | |(*d→g*) | ||
|EcoRI | |EcoRI | ||
| - | | | + | |7046bp, 18bp, 284bp |
| - | | | + | |Negative |
|- | |- | ||
|1D | |1D | ||
| Line 54: | Line 54: | ||
|(*d→g*) | |(*d→g*) | ||
|PstI | |PstI | ||
| - | | | + | |1155bp, 2511bp, 3682bp |
| - | | | + | |Negative |
|- | |- | ||
|1E | |1E | ||
| Line 61: | Line 61: | ||
|(*d→g*) | |(*d→g*) | ||
|PstI | |PstI | ||
| - | | | + | |1962bp, 1704bp, 3682bp |
| - | | | + | |Negative |
|- | |- | ||
|1F | |1F | ||
| Line 68: | Line 68: | ||
|(*d→g*) | |(*d→g*) | ||
|EcoRI | |EcoRI | ||
| - | | | + | |7046bp, 18bp, 284bp |
| - | | | + | |Negative |
|- | |- | ||
|1G | |1G | ||
| Line 75: | Line 75: | ||
|(*d→g*) | |(*d→g*) | ||
|PstI | |PstI | ||
| - | | | + | |1155bp, 2511bp, 3682bp |
| - | | | + | |Negative |
|- | |- | ||
|1H | |1H | ||
| Line 82: | Line 82: | ||
|(*d→g*) | |(*d→g*) | ||
|PstI | |PstI | ||
| - | | | + | |1962bp, 1704bp, 3682bp |
| - | | | + | |Negative |
|} | |} | ||
Revision as of 14:18, 10 September 2007
| https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg | Back To Glasgow's Main Page | Back To Glasgow's Wetlab Log |
|---|
| Protocols | References | Resources | Orders | Biobricks Used | Gels |
Contents |
Week 10
Monday 3st September 2007
-
Christine's plates from 31/08/07 all grew except for 1B. Overnights were set up from 7 colonies on each of the plates on Sunday, labelled 1A1→1H7, 49 in total as there were none from plate 1B. Christine, Maia and Maija miniprpped them and digested them as follows:
PCR tube Colony DNA is taken from Insert Enzyme Expected sizes Result 1A D (*a→d*) EcoRI 3455bp, 18bp, 3654bp Negative 1B D (*a→d*) PstI 274bp, 6553bp Negative 1C S (*d→g*) EcoRI 7046bp, 18bp, 284bp Negative 1D S (*d→g*) PstI 1155bp, 2511bp, 3682bp Negative 1E S (*d→g*) PstI 1962bp, 1704bp, 3682bp Negative 1F Q (*d→g*) EcoRI 7046bp, 18bp, 284bp Negative 1G Q (*d→g*) PstI 1155bp, 2511bp, 3682bp Negative 1H Q (*d→g*) PstI 1962bp, 1704bp, 3682bp Negative Tuesday 4nd September 2007
Wednesday 5rd September 2007
Thursday 6th September 2007
Friday 7th September 2007
https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg Back To
Glasgow's
Main PageBack To
Glasgow's
Wetlab Log
