Glasgow/Wetlab/Week10
From 2007.igem.org
(Difference between revisions)
(→Tuesday 4nd September 2007) |
(→Tuesday 4nd September 2007) |
||
| Line 90: | Line 90: | ||
<ol> | <ol> | ||
<li> | <li> | ||
| - | [[User:Christinemerrick|Christine]] realised that the site directed mutagenesis she had attempted on Thursday 30/08/07 was wrong. S and Q were meant to be tested for (*a→d*), and D was meant to be tested for (*d→g*) but S and Q were tested for (*d→g*), and D was tested for (*a→d*). This means the digests from 03/09/07 were also incorrect. However, all is not lost because the sequencing results for S and D came back today and they show that both S and D contain (*d→g*). | + | [[User:Christinemerrick|Christine]] realised that the site directed mutagenesis she had attempted on Thursday 30/08/07 was wrong. S and Q were meant to be tested for (*a→d*), and D was meant to be tested for (*d→g*) but S and Q were tested for (*d→g*), and D was tested for (*a→d*). This means the digests from 03/09/07 were also incorrect. However, all is not lost because the sequencing results for S and D came back today and they show that both S and D contain (*d→g*). This means that, theoretically, 1C, 1D and 1E from 03/09/07 should be positive, but this is not the case. |
=== Wednesday 5rd September 2007 === | === Wednesday 5rd September 2007 === | ||
Revision as of 14:34, 10 September 2007
| https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg | Back To Glasgow's Main Page | Back To Glasgow's Wetlab Log |
|---|
| Protocols | References | Resources | Orders | Biobricks Used | Gels |
Contents |
Week 10
Monday 3rd September 2007
-
Christine's plates from 31/08/07 all grew except for 1B. Overnights were set up from 7 colonies on each of the plates on Sunday, labelled 1A1→1H7, 49 in total as there were none from plate 1B. Christine, Maia and Maija miniprpped them and digested them as follows:
This table is incorrect, see 04/09/07.PCR tube Colony DNA is taken from Insert Enzyme Expected sizes Result 1A D (*a→d*) EcoRI 3455bp, 18bp, 3654bp Negative 1B D (*a→d*) PstI 274bp, 6553bp Negative 1C S (*d→g*) EcoRI 7046bp, 18bp, 284bp Negative 1D S (*d→g*) PstI 1155bp, 2511bp, 3682bp Negative 1E S (*d→g*) PstI 1962bp, 1704bp, 3682bp Negative 1F Q (*d→g*) EcoRI 7046bp, 18bp, 284bp Negative 1G Q (*d→g*) PstI 1155bp, 2511bp, 3682bp Negative 1H Q (*d→g*) PstI 1962bp, 1704bp, 3682bp Negative Tuesday 4nd September 2007
-
Christine realised that the site directed mutagenesis she had attempted on Thursday 30/08/07 was wrong. S and Q were meant to be tested for (*a→d*), and D was meant to be tested for (*d→g*) but S and Q were tested for (*d→g*), and D was tested for (*a→d*). This means the digests from 03/09/07 were also incorrect. However, all is not lost because the sequencing results for S and D came back today and they show that both S and D contain (*d→g*). This means that, theoretically, 1C, 1D and 1E from 03/09/07 should be positive, but this is not the case.
Wednesday 5rd September 2007
Thursday 6th September 2007
Friday 7th September 2007
https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg Back To
Glasgow's
Main PageBack To
Glasgow's
Wetlab Log
-
Christine realised that the site directed mutagenesis she had attempted on Thursday 30/08/07 was wrong. S and Q were meant to be tested for (*a→d*), and D was meant to be tested for (*d→g*) but S and Q were tested for (*d→g*), and D was tested for (*a→d*). This means the digests from 03/09/07 were also incorrect. However, all is not lost because the sequencing results for S and D came back today and they show that both S and D contain (*d→g*). This means that, theoretically, 1C, 1D and 1E from 03/09/07 should be positive, but this is not the case.
