Glasgow/Wetlab/Week7
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| pCR4 ||XylR & Pr || [https://2007.igem.org/Glasgow/Wetlab/Orders Pr_Prefix_1] & [https://2007.igem.org/Glasgow/Wetlab/Orders XylR_Suffix_1] | | pCR4 ||XylR & Pr || [https://2007.igem.org/Glasgow/Wetlab/Orders Pr_Prefix_1] & [https://2007.igem.org/Glasgow/Wetlab/Orders XylR_Suffix_1] | ||
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Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow. | Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow. | ||
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| pSB1AC3 (3/20G) || DntR || [https://2007.igem.org/Glasgow/Wetlab/Orders DntR_Prefix_1] & [https://2007.igem.org/Glasgow/Wetlab/Orders DntR_Suffix_2] | | pSB1AC3 (3/20G) || DntR || [https://2007.igem.org/Glasgow/Wetlab/Orders DntR_Prefix_1] & [https://2007.igem.org/Glasgow/Wetlab/Orders DntR_Suffix_2] | ||
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Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow. | Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow. | ||
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| - | [[User:Mojs|Maia]] took the following BioBricks from the kit plates and transformed into ''E. coli'' TOP10 cells: | + | [[User:Mojs|Maia]] took the following BioBricks from the kit plates and transformed (see [[Glasgow/Wetlab/Protocols#Protocol 2: Transforming BioBricks|Protocol 2]]) into ''E. coli'' TOP10 cells: |
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|[http://partsregistry.org/Part:BBa_E0432 Bba_E0432] || EYFP + RBS + terminator + LVA tag || ''E. coli'' TOP10 || pSB1A2 || 4/11C | |[http://partsregistry.org/Part:BBa_E0432 Bba_E0432] || EYFP + RBS + terminator + LVA tag || ''E. coli'' TOP10 || pSB1A2 || 4/11C | ||
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=== Tuesday 14th August 2007 === | === Tuesday 14th August 2007 === | ||
Revision as of 20:16, 15 August 2007
Back To Main Page | Back To Wetlab Log
Contents |
Week 7
Monday 13th August 2007
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Lynsey did colony PCR (see Protocol 9) on the following E. coli TOP10 transformants to check that their plasmids carry our expected inserts:
Plasmid Expected Insert Primers pCR4 Pu Pu_Prefix_After & Pu_Suffix_After pCR4 Pu Pu_Prefix_Emma & Pu_Suffix_Emma pCR4 Pr Pr_Prefix_1 & Pr_Suffix_1 pCR4 XylR XylR_Prefix_1 & XylR_Suffix_1 pCR4 XylR & Pr Pr_Prefix_1 & XylR_Suffix_1
Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow. -
Maija did colony PCR (see Protocol 9) on the following E. coli DB3.1 transformants to check that their plasmids carry our expected inserts:
Plasmid Expected Insert Primers pSB3K5 (4/6B) (*m*) B1 17 (*m*)_for_1 & BBs_Methyl_2 pSB1AC3 (3/20G) (*m*) B1 17 (*m*)_for_1 & BBs_Methyl_2 pSB3K5 (4/6B) (*m*) C5 24 (*m*)_for_1 & (*m*)_rev_1 pSB1AC3 (3/20G) (*m*) (*m*)_for_1 & (*m*)_rev_1 pSB3K5 (4/6B) DntR DntR_Prefix_1 & DntR_Suffix_2 pSB1AC3 (3/20G) DntR DntR_Prefix_1 & DntR_Suffix_2
Overnight cultures were seeded from colonies that gave successful PCR results in order to miniprep the plasmids tomorrow.
-
Maia took the following BioBricks from the kit plates and transformed (see Protocol 2) into E. coli TOP10 cells:
BioBrick Part Function Chassis Plasmid Kit Plate Location (Plate/Well) Bba_B0014 Double terminator E. coli TOP10 pSB1AK3 1/1G Bba_B0015 Double terminator E. coli TOP10 pSB1AK3 1/1I " " " " 3/3O Bba_J61100 RBS E. coli TOP10 pSB1A2 4/12N Bba_J61101 RBS E. coli TOP10 pSB1A2 4/12J Bba_J61102 RBS E. coli TOP10 pSB1A2 4/12L Bba_J61103 RBS E. coli TOP10 pSB1A2 4/12P Bba_E0430 EYFP + RBS + terminator E. coli TOP10 pSB1A2 1/11A Bba_E0422 ECFP + RBS + terminator + LVA tag E. coli TOP10 pSB1AK3 1/11G Bba_E0432 EYFP + RBS + terminator + LVA tag E. coli TOP10 pSB1A2 4/11C
Tuesday 14th August 2007
Wednesday 15th August 2007
Thursday 16th August 2007
=== Friday 17th August 2007 ===
