Glasgow/Wetlab/Week8

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Week 8

Monday 20th August 2007

  1. Chrsitine did more minipreps for colonies suspected of containing the 7 gene operon. When these minipreps were run on a gel there was no evidence of an insert.


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Tuesday 21st August 2007

  1. Maija digested the construction vectors 3/20G and 4/6B, and the 7 gene operon with SpeI and XbaI (Protocol 7) with the intention of then ligating the 7 gene operon into the construction vectors. The 7 gene operon DNA (taken from sample 2 of the PCR which was gel extracted 13/08/07) did not appear on the gel.
    Label Description Enzymes Expected size Result
    3/20G Construction vector SpeI, XbaI 2071bp 2071bp
    4/6B Construction vector SpeI, XbaI 2928bp 2928bp
    (*a→g*) 7 gene operon SpeI, XbaI 6263bp No result
  2. Christine gel extracted 4/6B and 3/20G (see Protocol 11) using 30ul ddH2O at 60°C to elute the DNA.

    Wednesday 22nd August 2007

    Thursday 23rd August 2007

    === Friday 24th August 2007 ===