Glasgow/Wetlab/Week9

From 2007.igem.org

(Difference between revisions)
(Monday 27th August 2007)
(Monday 27th August 2007)
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#[[User:MaijaP|Maija]] performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme.
#[[User:MaijaP|Maija]] performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme.
 +
{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
!Insert
 +
!Vector
 +
!Enzyme
 +
!Expected size
 +
!Result
 +
|-
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|pDntA
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|3/20G
 +
|PvuI
 +
|1450bp + 749bp
 +
|
 +
|-
 +
|pDntA
 +
|4/6B
 +
|PvuI
 +
|2375bp + 681bp
 +
|
 +
|}
#These
#These

Revision as of 14:03, 29 August 2007

Glasgow Main Page | Back To Wetlab Log


Protocols References Resources Orders Biobricks
Used
Gels

Contents

Week 9

Monday 27th August 2007

  1. Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme.
Insert Vector Enzyme Expected size Result
pDntA 3/20G PvuI 1450bp + 749bp
pDntA 4/6B PvuI 2375bp + 681bp
  1. These
  2. are
    1. numbered
  3. sections



This is a table
Look at the code to see the template.

Tuesday 28 August 2007

Wednesday 29th August 2007

Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. This will tell us if the BioBrick insert region is the expected (enlarged) size.

Thursday 30th August 2007

Friday 31st August 2007