Glasgow/Wetlab/Week9

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Week 9

Monday 27th August 2007

  1. Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands were weird sizes, see the gel here.
    Insert Vector Enzyme Expected size Result
    pDntA 3/20G PvuI 1450bp + 749bp ~2500bp
    pDntA 4/6B PvuI 2375bp + 681bp ~3800bp + ~1250bp
  2. [[User:Christinemerrick|Christine] digested minipreps of interest B, C and D suspected to contain (*dā†’g*) from 24/08/07 with PstI. Miniprep of colony D shows bands of expeceted sizes 1155bp, 549bp, 1962bp, and 3682bp.

    Tuesday 28 August 2007

    Wednesday 29th August 2007

    Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. Unfortunately it looks as though the combined product is larger than the sum of its parts...

    Scott also did the following restriction digests (see Protocol 7):

    Insert Digest 1 (EcoRI/PvuI) Insert Digest 2 (EcoRI/SpeI)
    (Done overnight at 16°C)
    J23119 (tube 1) J61101
    J23119 (tube 2) "


    Destination (EcoRI/XbaI)
    (Done overnight at 16°C)
    J61101


    Thursday 30th August 2007

    Friday 31st August 2007

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