Imperial/Cell by Date/Specification

From 2007.igem.org

< Imperial(Difference between revisions)
m
m (Cell by Date: Specifications)
 
(35 intermediate revisions not shown)
Line 1: Line 1:
{{Template:IC07navmenu}}
{{Template:IC07navmenu}}
 +
<br clear="all">
<html>
<html>
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" />
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" />
Line 22: Line 23:
'''Cell by Date in the Cold Chain:'''
'''Cell by Date in the Cold Chain:'''
-
<font color=red>--[[User:Anthony L|Anthony L]] 20:12, 25 October 2007 (EDT)Would like someone to come along and make me an image of same size 900x140 pixels describing in a cool fun way the cold chain and where cell by date fits in -> Mr.Lucas Mr . Jimmi </font>
 
-
[[Image:CBDCartoon.png]]
+
[[Image:cbd.png|800px]]
-
 
-
 
-
[[Image:IC07 spec sysresponse.png|thumb|400px|left|'''Desired response''' of Infector Detector. Note that the response is not necessarily linear.]]
 
-
* '''Input: AHL 5-50nM'''
 
-
It is known that, in Pseudomonas ''aeruginosa'' biofilms, the concentration of AHL is at least 5nM. <sup>[[#References|1-2]]</sup> Therefore, if the system can successfully report the presence of this concentration, it should be able to detect such biofilms.
 
-
* '''Output: Visible fluorescent protein'''
 
-
In order for the system to be used by a nurse, without needing any special equipment, the output signal must be visible.
 
-
 
-
 
-
== Specifications Table ==
 
-
 
-
{|border="1" width="80%" align="center"
 
-
|-
 
-
|width="20%" style="background:#ffffcc"|<center>'''Property'''</center>
 
-
|width="--"|<center>'''Value'''</center>
 
-
|-
 
-
|style="background:#ffffcc"|Inputs
 
-
|<center>Isothermal conditions between 0 & 40 &deg;C</center>
 
-
|-
 
-
|style="background:#ffffcc"|
 
-
|<center>Dynamic conditions eg. steps & ramps</center>
 
-
|-
 
-
|style="background:#ffffcc"|Outputs
 
-
|<center>System should give a visual signal to indicate level of thermal exposure where beef is off</center>
 
-
|-
 
-
|style="background:#ffffcc"|Activation Energy
 
-
|<center>System needs to have an activation Energy 30 +/- 20 kJ/mol</center>
 
-
|-
 
-
|style="background:#ffffcc"|Health Regulations
 
-
|<center>System must not be living replicating bacteria</center>
 
-
|-
 
-
|style="background:#ffffcc"|Response Time
 
-
|<center>System needs to have a response time under 1 hour</center>
 
-
|-
 
-
|style="background:#ffffcc"|Lifespan
 
-
|<center>System must have a shelf life of 7 days</center>
 
-
|}
 
<br clear="all">
<br clear="all">
-
== Project Plan ==
+
{|
-
===== Targetting Aerobically Fresh Ground Beef =====
+
|-valign="top"
-
The dominant organisms leading to the spoilage of beef depend of the beef's composition and the environmental conditions under which the beef is stored.  For refrigerated packaged beef Pseudomonas spp. were dominate areobically while Lactobacillus was dominant anaerobically.<sup>[[#References |1]]</sup> Because bacteria are responsible for the spoilage of beef it is unwise to use a bacteria based device eg. by using e.coli/yeast as a chassis as this would further add to the spoilage.
+
|width=10%|[[Image:CBDthermometer.gif|center]]<br><br><br><br><br><br><br><br>[[Image:CBDCartoonHamburger1.png|center]]<br clear ="all"><br><br><br><br><br><br>[[Image:CBDQuestionMarkCartoon.png|center]]<br>
 +
|width=30%|[[Image:CBDSpecs.png]]
 +
|width=60%|
 +
*'''Input: Isothermal & Dynamic conditions, eg. steps & ramps between 0-40 &deg;C'''
 +
Temperature is considered to be the major factor in beef spoilage and although the meat industry tries to keep temperature low during transportaion temperature conditions higher than 10&deg;C are not unusual during transport<sup>[[#References |3]]</sup>. It is therefore important that our that we look at the performance of our system in isothermal conditions eg. in the cold chain, and dynamic temperature scenarios eg. a break in the cold chain.
 +
*'''Output: Visible Signal'''
 +
Several Technologies already exist which address the problem of monitoring the thermal exposure of products in the cold chain.  One particular family are Temperature Time Integrators (TTIs).<sup>[[#References |5]]</sup><br>
 +
The key aspect of a TTI is that they are based on a phenomenon which can act as a signal to a consumer for example, eg. a colour change.  For Cell By Date we would like a visible signal that tells the consumer that as a result of the thermal history of the beef it is not fit for consumption e.g. it's been left out of the fridge for too long.  To Determine the point at which ground beef is not good to eat we have to look at the biology of the spoilage process.The dominant organisms leading to the spoilage of beef depend of the beef's composition and the environmental conditions under which the beef is stored.  For refrigerated packaged beef Pseudomonas spp. were dominate areobically while Lactobacillus was dominant anaerobically.<sup>[[#References |1]]</sup>  
 +
*'''Lifespan : 7 days'''
There also seems to be a general rule for beef that when the bacterial count reaches 10<sup>7</sup> cm<sup>-2</sup>, off odours and slime production occur and the beef is considered off. <sup>[[#References |2]]</sup> For controlled isothermal conditions in a laboratory environment the time taken for beef to reach this spoilage point seems to be at most 7 days.<sup>[[#References |3]]</sup> This implies that the shelf life of our system needs to be at least 7 days.
There also seems to be a general rule for beef that when the bacterial count reaches 10<sup>7</sup> cm<sup>-2</sup>, off odours and slime production occur and the beef is considered off. <sup>[[#References |2]]</sup> For controlled isothermal conditions in a laboratory environment the time taken for beef to reach this spoilage point seems to be at most 7 days.<sup>[[#References |3]]</sup> This implies that the shelf life of our system needs to be at least 7 days.
-
The Gompertz's model is widely used when considering beef spoilage as it has been shown to fit growth data very well. <sup>[[#References |1]]</sup>  Using the Gompertz model we can get the specific growth rate, Lag phase duration (LPD) and maximum population density (MPD) for bacterial growth at a  particular constant temperature.  And then using these we can determine the Activation Energy (Ea) for the beef spoilage reaction.  For U2 grade Argentinian beef stored in polyethylene and SARAN PVC, the Ea ranged from 80kJ mol<sup>-1</sup> to 220kJ mol<sup>-1</sup> for a range of bacteria. <sup>[[#References |4]]</sup>
+
*'''Health & Safety : Non-living , non-infectious'''<br>
 +
Because bacteria are responsible for the spoilage of beef it is unwise to use a bacteria based device eg. by using e.coli/yeast as a chassis as this would further add to the spoilage.
-
One contrary value for the Ea of the beef spoilage rxns is given by Leak <sup>[[#References |2]]</sup> who calculated Ea = 30kJ mol<sup>-1</sup>.  The difference between Leak's value and that of Giannuzzi <sup>[[#References |4]]</sup> probably lies in their packaging methods, which is of importance as the project aims to target aerobically fresh gound beef.
+
*'''Activation Energy : 30 +/- 20 kJ/mol'''<br>
-
===== A Biological Temperature Time Integrator =====
+
The Gompertz's model is widely used when considering beef spoilage as it has been shown to fit growth data very well. <sup>[[#References |1]]</sup>  Using the Gompertz model we can get the specific growth rate, Lag phase duration (LPD) and maximum population density (MPD) for bacterial growth at a  particular constant temperature. And then using these we can determine the Activation Energy (Ea) for the beef spoilage reaction.  For U2 grade Argentinian beef stored in polyethylene and SARAN PVC, the Ea ranged from 80kJ mol<sup>-1</sup> to 220kJ mol<sup>-1</sup> for a range of bacteria. <sup>[[#References |4]]</sup>
-
Temperature is considered to be the major factor in beef spoilage and although industry tries to keep temperature low during transportaion exposure of the beef to 10 degrees celcius are not unusual. It is therefore important that our that we look at the performance of our system in isothermal conditions eg. in the cold chain, and dynamic temperature scenarios eg. a break in the cold chain.
+
-
Several Technologies have already been developed to address the problem of monitoring the thermal exposure of products in the cold chainOne particular family of these products are called Temperature Time Integrators (TTI).<sup>[[#References |5]]</sup>
+
One contrary value for the Ea of the beef spoilage reactions is given by Leak <sup>[[#References |2]]</sup> who calculated Ea = 30kJ mol<sup>-1</sup>The difference between Leak's value and that of Giannuzzi <sup>[[#References |4]]</sup> probably lies in their packaging methods, which is of importance as the project aims to target aerobically fresh gound beef.<br>
-
The key aspect of a TTI is that they are based on a phenomenon which can act as a signal to a consumer for example, eg. a colour change.  The rate at which this change occours needs to be temperature dependant so it can mimic the effect temperature has on the spoiling of meat eg. change happens quicker at higher temperatures.  In order for a TTI to accurately report the spoilage rxn of beef, the activation energy of the two rxns needs to be similar.  For example a difference between the two Ea's less than 20kJ mol<sup>-1</sup> would result in the TTI estimating the thermal history of the beef to be within 1 degree C of the actual history.<sup>[[#References |6]]</sup>
+
In order for Cell by Date to act as a TTI & accurately report the spoilage rxn of beef, the activation energy of the two rxns needs to be similar.  For example a difference between the two Ea's less than 20kJ mol<sup>-1</sup> would result in the TTI estimating the thermal history of the beef to be within 1&deg;C of the actual history.<sup>[[#References |6]]</sup> For Cell by Date we would like our Activation Energy of our system to be 30 +/- 20 kJ mol<sup>-1</sup> to yield an accurate thermal history of the most sensitive spoilage rxn found by Leak, which is most likely for aerobically fresh beef.
 +
*'''Response Time: Less that 1 Hour'''<br>
 +
In addition, to correctly couple the Ea of our system to that of the dominant spoilage reaction in beef, we also have to consider the response time of our sytem.  Our system needs to have a rapid response time, so in other words it needs to be able to quickly switch between states eg. low output and high output.  This is so our system can capture small variations of temperature in the cold chain and report them in a meangingful way.  Specifically, a response time in the order of a few hours would ensure that if there are any problems in the cold chain this will arise in our system very quickly.
 +
|}
-
Applying this to our TTI this would mean that the Activation Energy of our system needs to be 30 +/- 20 kJ mol<sup>-1</sup> if we are considering the most easily achieved activation energy found by Leak, which is most likely for aerobically fresh beef.
 
-
 
-
In addition, to correctly coupld the Ea of our system to that of the dominant spoilage reaction in beef, we also have to consider the response time of our sytem.  Our system needs to have a rapid response time, so in other words it needs to be able to quickly switch between states eg. low output and high output.  This is so our system can capture small variations of temperature in the cold chain and report them in a meangingful way.  Specifically, a response time in the order of a few hours would ensure that if there are any problems in the cold chain this will arise in our system very quickly.
 
Line 94: Line 65:
# [http://www.springerlink.com/index/G311226U2M51T844.pdf Labuza TP, Fu B. Growth kinetics for shelf-life prediction: theory and practice. J of Industrial Microbiology 1993;12:309-23.]
# [http://www.springerlink.com/index/G311226U2M51T844.pdf Labuza TP, Fu B. Growth kinetics for shelf-life prediction: theory and practice. J of Industrial Microbiology 1993;12:309-23.]
# [http://66.102.1.104/scholar?hl=en&lr=&q=cache:thi6BTIW1YMJ:www.vitsab.com/PDF/V507.pdf+TTI+Beef+Activation+Energy Leak, F.W. (2000): Quality changes in Ground beef during distribution and storage, and determination of Time- Temperature-Indicator (TTI) charakteristic of ground beef University of Florida Institute of food and Agricultural Sciences Internet: www.vitsab.com, Stand: April 2003]
# [http://66.102.1.104/scholar?hl=en&lr=&q=cache:thi6BTIW1YMJ:www.vitsab.com/PDF/V507.pdf+TTI+Beef+Activation+Energy Leak, F.W. (2000): Quality changes in Ground beef during distribution and storage, and determination of Time- Temperature-Indicator (TTI) charakteristic of ground beef University of Florida Institute of food and Agricultural Sciences Internet: www.vitsab.com, Stand: April 2003]
-
# [http://iufost.edpsciences.org/index.php?option=article&access=standard&Itemid=129&url=/articles/iufost/pdf/2006/01/iufost06000765.pdf Koutsoumanis K, Stamatiou A, Skandamis P, Nychas GJ. Development of a Microbial Model for the Combined Effect of Temperature and pH on Spoilage of Ground Meat, and Validation of the Model under Dynamic Temperature Conditions. Appl Environ Microbiol. 2006 Jan;72(1):124-34.]
+
# [http://aem.asm.org/cgi/content/abstract/72/1/124 Koutsoumanis K, Stamatiou A, Skandamis P, Nychas GJ. Development of a Microbial Model for the Combined Effect of Temperature and pH on Spoilage of Ground Meat, and Validation of the Model under Dynamic Temperature Conditions. Appl Environ Microbiol. 2006 Jan;72(1):124-34.]
# [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-3S3M25D-B&_user=217827&_coverDate=01%2F06%2F1998&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=80630ba21fbc6869b9f5179d334734da Giannuzzi L, Pinotti A, Zaritzky N. Mathematical modelling of microbial growth in packaged refrigerated beef stored at different temperatures. Int J Food Microbiol. 1998 Jan 6;39(1-2):101-10.]
# [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7K-3S3M25D-B&_user=217827&_coverDate=01%2F06%2F1998&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=80630ba21fbc6869b9f5179d334734da Giannuzzi L, Pinotti A, Zaritzky N. Mathematical modelling of microbial growth in packaged refrigerated beef stored at different temperatures. Int J Food Microbiol. 1998 Jan 6;39(1-2):101-10.]
# [http://www.iaph.uni-bonn.de/Coldchain/downloads/06_Labuza.pdf Labuza 2006 : Cold Chain-Management II Time-temperature Integrators and the Cold chain: What is next? Bonn Germany 5/8/06]<br>
# [http://www.iaph.uni-bonn.de/Coldchain/downloads/06_Labuza.pdf Labuza 2006 : Cold Chain-Management II Time-temperature Integrators and the Cold chain: What is next? Bonn Germany 5/8/06]<br>
# [http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-2621.2001.tb15211.x?cookieSet=1 E. Shimoni, E.M. Anderson, T.P. Labuza (2001) Reliability of Time Temperature Indicators Under Temperature Abuse. Journal of Food Science 66 (9), 1337–1340. doi:10.1111/j.1365-2621.2001.tb15211.x ]
# [http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-2621.2001.tb15211.x?cookieSet=1 E. Shimoni, E.M. Anderson, T.P. Labuza (2001) Reliability of Time Temperature Indicators Under Temperature Abuse. Journal of Food Science 66 (9), 1337–1340. doi:10.1111/j.1365-2621.2001.tb15211.x ]
# [http://linkinghub.elsevier.com/retrieve/pii/S0168-1605(05)00067-X Giannakourou MC, Koutsoumanis K, Nychas GJ, Taoukis PS. Field evaluation of the application of time temperature integrators for monitoring fish quality in the chill chain. Int J Food Microbiol. 2005 Jul 25;102(3):323-36.]
# [http://linkinghub.elsevier.com/retrieve/pii/S0168-1605(05)00067-X Giannakourou MC, Koutsoumanis K, Nychas GJ, Taoukis PS. Field evaluation of the application of time temperature integrators for monitoring fish quality in the chill chain. Int J Food Microbiol. 2005 Jul 25;102(3):323-36.]

Latest revision as of 03:22, 27 October 2007




Cell by Date: Specifications

Cell by Date in the Cold Chain:


Cbd.png


CBDthermometer.gif








CBDCartoonHamburger1.png







CBDQuestionMarkCartoon.png

CBDSpecs.png
  • Input: Isothermal & Dynamic conditions, eg. steps & ramps between 0-40 °C

Temperature is considered to be the major factor in beef spoilage and although the meat industry tries to keep temperature low during transportaion temperature conditions higher than 10°C are not unusual during transport3. It is therefore important that our that we look at the performance of our system in isothermal conditions eg. in the cold chain, and dynamic temperature scenarios eg. a break in the cold chain.

  • Output: Visible Signal

Several Technologies already exist which address the problem of monitoring the thermal exposure of products in the cold chain. One particular family are Temperature Time Integrators (TTIs).5
The key aspect of a TTI is that they are based on a phenomenon which can act as a signal to a consumer for example, eg. a colour change. For Cell By Date we would like a visible signal that tells the consumer that as a result of the thermal history of the beef it is not fit for consumption e.g. it's been left out of the fridge for too long. To Determine the point at which ground beef is not good to eat we have to look at the biology of the spoilage process.The dominant organisms leading to the spoilage of beef depend of the beef's composition and the environmental conditions under which the beef is stored. For refrigerated packaged beef Pseudomonas spp. were dominate areobically while Lactobacillus was dominant anaerobically.1

  • Lifespan : 7 days

There also seems to be a general rule for beef that when the bacterial count reaches 107 cm-2, off odours and slime production occur and the beef is considered off. 2 For controlled isothermal conditions in a laboratory environment the time taken for beef to reach this spoilage point seems to be at most 7 days.3 This implies that the shelf life of our system needs to be at least 7 days.

  • Health & Safety : Non-living , non-infectious

Because bacteria are responsible for the spoilage of beef it is unwise to use a bacteria based device eg. by using e.coli/yeast as a chassis as this would further add to the spoilage.

  • Activation Energy : 30 +/- 20 kJ/mol

The Gompertz's model is widely used when considering beef spoilage as it has been shown to fit growth data very well. 1 Using the Gompertz model we can get the specific growth rate, Lag phase duration (LPD) and maximum population density (MPD) for bacterial growth at a particular constant temperature. And then using these we can determine the Activation Energy (Ea) for the beef spoilage reaction. For U2 grade Argentinian beef stored in polyethylene and SARAN PVC, the Ea ranged from 80kJ mol-1 to 220kJ mol-1 for a range of bacteria. 4

One contrary value for the Ea of the beef spoilage reactions is given by Leak 2 who calculated Ea = 30kJ mol-1. The difference between Leak's value and that of Giannuzzi 4 probably lies in their packaging methods, which is of importance as the project aims to target aerobically fresh gound beef.

In order for Cell by Date to act as a TTI & accurately report the spoilage rxn of beef, the activation energy of the two rxns needs to be similar. For example a difference between the two Ea's less than 20kJ mol-1 would result in the TTI estimating the thermal history of the beef to be within 1°C of the actual history.6 For Cell by Date we would like our Activation Energy of our system to be 30 +/- 20 kJ mol-1 to yield an accurate thermal history of the most sensitive spoilage rxn found by Leak, which is most likely for aerobically fresh beef.

  • Response Time: Less that 1 Hour

In addition, to correctly couple the Ea of our system to that of the dominant spoilage reaction in beef, we also have to consider the response time of our sytem. Our system needs to have a rapid response time, so in other words it needs to be able to quickly switch between states eg. low output and high output. This is so our system can capture small variations of temperature in the cold chain and report them in a meangingful way. Specifically, a response time in the order of a few hours would ensure that if there are any problems in the cold chain this will arise in our system very quickly.


<< Introduction | Specifications | Design >>


References

  1. Labuza TP, Fu B. Growth kinetics for shelf-life prediction: theory and practice. J of Industrial Microbiology 1993;12:309-23.
  2. Leak, F.W. (2000): Quality changes in Ground beef during distribution and storage, and determination of Time- Temperature-Indicator (TTI) charakteristic of ground beef University of Florida Institute of food and Agricultural Sciences Internet: www.vitsab.com, Stand: April 2003
  3. Koutsoumanis K, Stamatiou A, Skandamis P, Nychas GJ. Development of a Microbial Model for the Combined Effect of Temperature and pH on Spoilage of Ground Meat, and Validation of the Model under Dynamic Temperature Conditions. Appl Environ Microbiol. 2006 Jan;72(1):124-34.
  4. Giannuzzi L, Pinotti A, Zaritzky N. Mathematical modelling of microbial growth in packaged refrigerated beef stored at different temperatures. Int J Food Microbiol. 1998 Jan 6;39(1-2):101-10.
  5. Labuza 2006 : Cold Chain-Management II Time-temperature Integrators and the Cold chain: What is next? Bonn Germany 5/8/06
  6. E. Shimoni, E.M. Anderson, T.P. Labuza (2001) Reliability of Time Temperature Indicators Under Temperature Abuse. Journal of Food Science 66 (9), 1337–1340. doi:10.1111/j.1365-2621.2001.tb15211.x
  7. Giannakourou MC, Koutsoumanis K, Nychas GJ, Taoukis PS. Field evaluation of the application of time temperature integrators for monitoring fish quality in the chill chain. Int J Food Microbiol. 2005 Jul 25;102(3):323-36.