Imperial/Wet Lab/Lab Notebook/2007-09-02

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< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
(2 September 2007)
 
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= 2 September 2007 =
= 2 September 2007 =
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==Construction of pT7-GFP==
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#5 colonies grew in the ligation plate
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#All 5 colonies were picked and each was grown in 5 ml of LB + Kan
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#The 5 cultures were incubated at 37&deg;C overnight
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==Cloning of Biobricks (for Maxiprep)==
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#Placed 200 ml of LB in a 500 ml conical flask
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#Innoculated 2 ml of culture into LB media in flask
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#Incubated overnight at 37 °C
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<font size=-2>
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*2. BBa_I13422 [ptet-GFP]
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*18. BBa_T9002 [plux-GFP]
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</font>
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Latest revision as of 14:06, 10 October 2007

2 September 2007

Construction of pT7-GFP

  1. 5 colonies grew in the ligation plate
  2. All 5 colonies were picked and each was grown in 5 ml of LB + Kan
  3. The 5 cultures were incubated at 37°C overnight

Cloning of Biobricks (for Maxiprep)

  1. Placed 200 ml of LB in a 500 ml conical flask
  2. Innoculated 2 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 2. BBa_I13422 [ptet-GFP]
  • 18. BBa_T9002 [plux-GFP]