July 1

From 2007.igem.org

(Difference between revisions)
(Experiments)
(Experiments)
 
Line 16: Line 16:
* Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
* Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM
i) for FACS (in sets of three)
i) for FACS (in sets of three)
 +
ii) for Microscopy (in sets of two).
ii) for Microscopy (in sets of two).

Latest revision as of 12:30, 3 July 2007

Back to Bangalore

Back to e-Notebook

Experiments

  • pL.LuxI.C was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
  • pL.LuxR.Y was inoculated in 3 mL L/B and 3 uL 100 mg/mL Amp for equivalence expt to be done on July 2.
  • Dilutions of pL.LuxI.C made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM

i) for FACS (in triplicate for a particular inoculum volume)

ii) for Microscopy (for 2 inoculum volumes: 0.1 & 0.01 uL/mL)

  • Dilutions of pL.LuxR.Y made in M9 with IPTG conc 0,5,10,50,100,500,1000 uM

i) for FACS (in sets of three)

ii) for Microscopy (in sets of two).