# July 3

(Difference between revisions)
 Revision as of 09:39, 7 July 2007 (view source)Vgautam (Talk | contribs) (→Analysis)← Older edit Latest revision as of 05:06, 9 July 2007 (view source)Skundu (Talk | contribs) (→Analysis) (8 intermediate revisions not shown) Line 43: Line 43: ==Analysis== ==Analysis== - * We obtained the following scatter plots (mean log) showing the equivalence curves: + * We obtained the following scatter plots (mean log) for the equivalence curves: - + {|align="center" width="75%" - + !align="center"|pL.luxR.YFP - {|align="center" width="85%" + !align="center"|pT.luxI.CFP |- |- - |[[Image:File.jpg|thumb|300px|mean YFP vs IPTG]] + |[[Image:pLluxRY_0703_cfp.png|center|thumb|300px|mean CFP vs IPTG conc.]] - |[[Image:File2.jpg|thumb|300px|mean CFP vs IPTG]] + |[[Image:pTluxIC_0703_cfp.png|center|thumb|300px|mean CFP vs IPTG conc.]] |- |- + |[[Image:pLluxRY_0703_yfp.png|center|thumb|300px|mean YFP vs IPTG conc.]] + |[[Image:pTluxIC_0703_yfp.png|center|thumb|300px|mean YFP vs IPTG conc.]] + |- + |colspan="2" align="justify"|'''Scatter plot:''' Each dot here represents the fluorescence value of an individual cell. The Cyan dots represent the fluorescence values of wild-strain K12z1 cells. |} |} - + == Inference == - + - '''Inference''' +

## Latest revision as of 05:06, 9 July 2007

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## Experiments

• Open loop experiment [E]

i) Cells were transferred to 50 mL Glu M9 in 250 mL flask with [aTc]=50 ng/mL and inoculum=1,0.1 uL/mL.

ii) pL.LuxR.Y.pR.C and K12Z1 were inoculated in LB.

iii) K12z1 was inoculated in Glu M9 and atc (1000)

iv) The contents of the flask with required OD (50 ng/mL) were filtered.

v) Seven dilutions for pL.LuxR.Y.pR.C were made with 50 old:50 new 2xM9 along with -ve control: K12z1

• The following -ve controls were also done :

i) pL.LuxR.Y.pR.C with K12Z1 grown medium,500 uM IPTG.

ii) pL.LuxR.Y.pR.C with new M9,500 uM IPTG.

• Open loop experiment [F]

i) Cells were transferred to 50 mL Glu M9 in 250 mL flask with [aTc]=0 ng/mL and inoculum=1,0.1 uL/mL

ii) pL.LuxR.Y.pR.C, K12Z1 and pL.YFP were inoculated in LB

iii) The contents of the flask with required OD ( ) were filtered.

Microscopy

• pT.LuxI.C imaging was done as part of the equivalence expt.
• pL.LuxR.Y imaging was done as part of the eqiuvalence expt.

## Analysis

• We obtained the following scatter plots (mean log) for the equivalence curves:
pL.luxR.YFP pT.luxI.CFP
mean CFP vs IPTG conc.
mean CFP vs IPTG conc.
mean YFP vs IPTG conc.
mean YFP vs IPTG conc.
Scatter plot: Each dot here represents the fluorescence value of an individual cell. The Cyan dots represent the fluorescence values of wild-strain K12z1 cells.