July 6

From 2007.igem.org

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==Analysis==
==Analysis==
 +
 +
Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml).
 +
The following matrix of information was obtained:
 +
{|cellspacing="2" cellpadding="2" border="1" align="left"
 +
|-align="center"
 +
!colspan="2" rowspan="2"|
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CFP mean values
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|colspan="7"|
 +
IPTG (uM) or luxR.YFP
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|-align="center"
 +
| 0
 +
| 5
 +
| 10
 +
| 50
 +
| 100
 +
| 500
 +
|1000
 +
|-align="center"
 +
|rowspan="6"|
 +
aTc (ng/ml)
 +
 +
or
 +
 +
luxI.CFP
 +
|0
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|1
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
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|5
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|10
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
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|-align="center"
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|20
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
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|-align="center"
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|50
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|
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|
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|
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|
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|
 +
|
 +
|
 +
|}
 +
{|cellspacing="2" cellpadding="2" border="1" align="right"
 +
|-align="center"
 +
!colspan="2" rowspan="2"|
 +
YFP mean values
 +
|colspan="7"|
 +
IPTG (uM) or luxR.YFP
 +
|-align="center"
 +
| 0
 +
| 5
 +
| 10
 +
| 50
 +
| 100
 +
| 500
 +
|1000
 +
|-align="center"
 +
|rowspan="6"|
 +
aTc (ng/ml)
 +
 +
or
 +
 +
luxI.CFP
 +
|0
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|1
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|5
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|10
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|20
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|-align="center"
 +
|50
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|
 +
|
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|
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|
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|
 +
|
 +
|
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|}
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{|align="center" width="35%"
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|-
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|align="justified"|
 +
These values give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.
 +
 +
These values are those obtained from the microscopy experiments.
 +
 +
A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.
 +
|}

Revision as of 05:18, 7 July 2007

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Experiments

Analysis

Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:

CFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50

YFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50

These values give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.

These values are those obtained from the microscopy experiments.

A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.

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