July 6

From 2007.igem.org

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(Analysis)
(Analysis)
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Revision as of 05:57, 7 July 2007

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Experiments

Analysis

Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:

CFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50

YFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50
These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.

A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.

  • Red : 0 ng/ml aTc
  • Green : 1 ng/ml aTc
  • Blue : 5 ng/ml aTc
  • Cyan : 10 ng/ml aTc
  • Magenta : 20 ng/ml aTc
  • Yellow : 50 ng/ml aTc


CFP fluorescence
YFP fluorescence