July 6

From 2007.igem.org

(Difference between revisions)
(Analysis)
(Analysis)
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{|align="center" width="65%" border="1"
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!CFP Fluorescence
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!YFP Fluorescence
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|align="center"|[[Image:Openloop_cfp_jul6.jpg|thumb|300px|CFP fluorescence]]
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|align="center"|[[Image:Openloop_cfp_jul6.jpg|400px|CFP fluorescence]]
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|align="center"|[[Image:Openloop_yfp_jul6.jpg|thumb|300px|YFP fluorescence]]
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|align="center"|[[Image:Openloop_yfp_jul6.jpg|400px|YFP fluorescence]]
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|align="center"|[[Image:surf_plot_cfp_jul6.jpg|thumb|300px|CFP fluorescence]]
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|align="center"|[[Image:surf_plot_cfp_jul6.jpg|400px|CFP fluorescence]]
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|align="center"|[[Image:surf_plot_yfp_jul6.jpg|thumb|300px|YFP fluorescence]]
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|align="center"|[[Image:surf_plot_yfp_jul6.jpg|400px|YFP fluorescence]]
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|colspan="2" align="justify"|The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG.
|colspan="2" align="justify"|The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG.
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Revision as of 06:29, 7 July 2007

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Experiments

Analysis

Today we compiled the data collected from all our open-loop experiments performed at various concentrations of aTc (ng/ml). The following matrix of information was obtained:

CFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50

YFP mean values

IPTG (uM) or luxR.YFP

0 5 10 50 100 500 1000

aTc (ng/ml)

or

luxI.CFP

0
1
5
10
20
50
These values, obtained from the microscopy experiments, give us a clear idea of the equivalence between luxI.CFP and luxI.YFP.

A scatter plot with each dot representing a cell was also plotted to see the variation of fluorescence with the change in aTc and IPTG concentrations.

  • Red : 0 ng/ml aTc
  • Green : 1 ng/ml aTc
  • Blue : 5 ng/ml aTc
  • Cyan : 10 ng/ml aTc
  • Magenta : 20 ng/ml aTc
  • Yellow : 50 ng/ml aTc


CFP Fluorescence YFP Fluorescence
CFP fluorescence YFP fluorescence
CFP fluorescence YFP fluorescence
The increasing values along the luxI.CFP axis are the increasing concentrations of aTc whereas the increasing values along the luxR.YFP axis are the increasing concentrations of IPTG.