June 13
From 2007.igem.org
(Difference between revisions)
(→Experiments) |
|||
| (2 intermediate revisions not shown) | |||
| Line 14: | Line 14: | ||
* Negative control (K12Z1) in three sets (after 5 hrs). | * Negative control (K12Z1) in three sets (after 5 hrs). | ||
| - | * 0.01,0.1 and 1 uL of inoculated pL | + | * 0.01,0.1 and 1 uL of inoculated pL luxI cfp inoculated in M9 in sets of three : |
| - | + | {| align="center" | |
| + | |- | ||
| + | | | ||
| + | M9+ 1000 uM IPTG (1 mL) | ||
| + | M9+ 50 ng/mL ATC (1 mL) | ||
| + | |} | ||
| + | '''Microscopy''' | ||
| + | * pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs. | ||
| + | |||
| + | * K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs. | ||
| - | |||
| - | + | '''Note:''' There was a problem in the Flow Cytometer (FACS) sheath pressure and the filters were found misaligned. Thus fluorescence readings were not proper and we would take the FACS readings again. | |
| - | + | ||
| - | + | ||
Latest revision as of 12:14, 29 June 2007
Back to Bangalore
Back to e-Notebook
Experiments
- Flow cytometry carried out.
- pL CFP (J22202) induced with IPTG for 0,1,10,50,100,1000 uM after 5 hours in sets of three.
- pL CFP (J22202) induced with ATc for 0,1,10,25,50,100 ng/mL after 4 hrs in sets of three.
- pL YFP (J22203) induced with IPTG for 1000uM in three sets after 4 hrs (positive control).
- Negative control (K12Z1) in three sets (after 5 hrs).
- 0.01,0.1 and 1 uL of inoculated pL luxI cfp inoculated in M9 in sets of three :
M9+ 1000 uM IPTG (1 mL) M9+ 50 ng/mL ATC (1 mL) |
Microscopy
- pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
- K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
Note: There was a problem in the Flow Cytometer (FACS) sheath pressure and the filters were found misaligned. Thus fluorescence readings were not proper and we would take the FACS readings again.
