June 13

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* K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
* K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
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'''Note:''' There was a problem in the Flow Cytometer (FACS) sheath pressure and the filters were found misaligned. Thus fluorescence readings were not proper and we would take the FACS readings again.

Revision as of 10:47, 28 June 2007

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Experiments

  • Flow cytometry carried out.
  • pL CFP (J22202) induced with IPTG for 0,1,10,50,100,1000 uM after 5 hours in sets of three.
  • pL CFP (J22202) induced with ATc for 0,1,10,25,50,100 ng/mL after 4 hrs in sets of three.
  • pL YFP (J22203) induced with IPTG for 1000uM in three sets after 4 hrs (positive control).
  • Negative control (K12Z1) in three sets (after 5 hrs).
  • 0.01,0.1 and 1 uL of inoculated pL luxI cfp inoculated in M9 : M9+ 1000 uM IPTG (1 mL) 
                                              [X 3]              M9+ 50 ng/mL ATC  (1 mL)


Microscopy

  • pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
  • K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.


Note: There was a problem in the Flow Cytometer (FACS) sheath pressure and the filters were found misaligned. Thus fluorescence readings were not proper and we would take the FACS readings again.

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