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Experiments

• Flow cytometry carried out.

• pL CFP (J22202) induced with IPTG for 0,1,10,50,100,1000 uM after 5 hours in sets of three.

• pL CFP (J22202) induced with ATc for 0,1,10,25,50,100 ng/mL after 4 hrs in sets of three.

• pL YFP (J22203) induced with IPTG for 1000uM in three sets after 4 hrs (positive control).

• Negative control (K12Z1) in three sets (after 5 hrs).

• 0.01,0.1 and 1 uL of inoculated pL LuxI CFP diluted in M9: M9+ 1000 uM IPTG (1 ml)

                                                             M9+ 50 ng/mL ATC  (1 mL)

Microscopy

• pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.

• K12Z1 also induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.