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June 16 - Revision history
2024-03-29T09:31:46Z
Revision history for this page on the wiki
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Vgautam: /* Experiments */
2007-06-28T07:17:57Z
<p><span class="autocomment">Experiments</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 07:17, 28 June 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Inoculation''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Inoculation''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>pLac cfp was inoculated with <del class="diffchange diffchange-inline">different </del>IPTG induction levels at 0,1,10,50,100,1000 uM along with K12Z1 <del class="diffchange diffchange-inline">(</del>control<del class="diffchange diffchange-inline">)</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>pLac cfp was inoculated with IPTG induction levels at 0,1,10,50,100,1000 uM along with K12Z1 <ins class="diffchange diffchange-inline">as </ins>control.</div></td></tr>
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Vgautam
http://2007.igem.org/wiki/index.php?title=June_16&diff=4974&oldid=prev
Vgautam: /* Experiments */
2007-06-28T07:16:34Z
<p><span class="autocomment">Experiments</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 07:16, 28 June 2007</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==Experiments ==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''Back to [[Bangalore]]''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">Check </del>transformants <del class="diffchange diffchange-inline">--- Varun (Contamination </del>in negative<del class="diffchange diffchange-inline">, </del>lawn growth in rest<del class="diffchange diffchange-inline">)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''Back to [[e-Notebook]]''</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*Image </del>pLac cfp <del class="diffchange diffchange-inline">(9 AM onwards) (12,14 and 16 hrs)--- Navneet and Varun (Redo dilutions and image on 17th)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== Experiments ==</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*FACS (9 AM onwards)--- Krishna</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*</del>IPTG induction<del class="diffchange diffchange-inline">(pLac cfp) </del>at 0,1,10,50,100,1000 uM<del class="diffchange diffchange-inline">- (X 2) </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">The </ins>transformants <ins class="diffchange diffchange-inline">were checked and contamination was found </ins>in <ins class="diffchange diffchange-inline">the </ins>negative <ins class="diffchange diffchange-inline">control and </ins>lawn growth in rest <ins class="diffchange diffchange-inline">of the samples. Thus imaging could not be done.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*</del>K12Z1 (<del class="diffchange diffchange-inline">10 PM) (X 2)--- Navneet (11 PM</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''Inoculation''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>pLac cfp <ins class="diffchange diffchange-inline">was inoculated with different </ins>IPTG induction <ins class="diffchange diffchange-inline">levels </ins>at 0,1,10,50,100,1000 uM <ins class="diffchange diffchange-inline">along with </ins>K12Z1 (<ins class="diffchange diffchange-inline">control</ins>)<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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Vgautam
http://2007.igem.org/wiki/index.php?title=June_16&diff=4202&oldid=prev
Sreenivasan at 11:58, 21 June 2007
2007-06-21T11:58:44Z
<p></p>
<p><b>New page</b></p><div>==Experiments ==<br />
<br />
*Check transformants --- Varun (Contamination in negative, lawn growth in rest)<br />
*Image pLac cfp (9 AM onwards) (12,14 and 16 hrs)--- Navneet and Varun (Redo dilutions and image on 17th)<br />
*FACS (9 AM onwards)--- Krishna<br />
*IPTG induction(pLac cfp) at 0,1,10,50,100,1000 uM- (X 2) <br />
*K12Z1 (10 PM) (X 2)--- Navneet (11 PM)</div>
Sreenivasan