June 18

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(Experiments)
(Experiments)
 
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* pLac cfp Inoculated  in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening).
* pLac cfp Inoculated  in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening).
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* The new constructs viz.                               were transformed.
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* The new constructs viz. j22261,j22271,j22281,j22301 were transformed.
* pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
* pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
* pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
* pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
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* For FACS recalibration another set of pLac cfp was induced with IPTG levels of 0,10,100,1000 uM.

Latest revision as of 08:36, 28 June 2007

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Experiments

  • pLac yfp and pLac luxI cfp could not be imaged because the OD was found to be greater than 1.
  • pLac yfp inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (morning).
  • pLac cfp Inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening).
  • The new constructs viz. j22261,j22271,j22281,j22301 were transformed.
  • pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
  • pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
  • For FACS recalibration another set of pLac cfp was induced with IPTG levels of 0,10,100,1000 uM.