June 20
From 2007.igem.org
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== Experiments == | == Experiments == | ||
| - | Microscopy: | + | '''Microscopy:''' |
* pT luxI Cfp imaging done at various induction levels but OD was too high | * pT luxI Cfp imaging done at various induction levels but OD was too high | ||
* K12Z1 imaging done at various induction levels | * K12Z1 imaging done at various induction levels | ||
* 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2)) | * 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2)) | ||
| - | FACS: | + | '''FACS:''' |
* Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets | * Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets | ||
* pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets | * pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets | ||
* Fluorescence due to M9 solution (Control) | * Fluorescence due to M9 solution (Control) | ||
| - | |||
== Analysis == | == Analysis == | ||
Revision as of 04:28, 25 June 2007
Experiments
Microscopy:
- pT luxI Cfp imaging done at various induction levels but OD was too high
- K12Z1 imaging done at various induction levels
- 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))
FACS:
- Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
- pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
- Fluorescence due to M9 solution (Control)
Analysis
pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn
Red : K12Z1 Fluorescence values at different aTc induction levels Green : pT.luxI.C Fluorescence values at different aTc induction levels Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels
Linear and log space gallery
