June 20
From 2007.igem.org
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== Analysis == | == Analysis == | ||
| + | '''Microscopy:''' | ||
| + | |||
pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn | pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn | ||
| - | Red : K12Z1 Fluorescence values at different aTc induction levels | + | <font color="red">Red</font> : K12Z1 Fluorescence values at different aTc induction levels |
| - | Green : pT.luxI.C Fluorescence values at different aTc induction levels | + | |
| - | Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels | + | <font color="green">Green</font> : pT.luxI.C Fluorescence values at different aTc induction levels |
| + | |||
| + | <font color="blue">Blue</font> : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels | ||
Linear and log space | Linear and log space | ||
| + | |||
gallery | gallery | ||
Revision as of 04:32, 25 June 2007
Experiments
Microscopy:
- pT luxI Cfp imaging done at various induction levels but OD was too high
- K12Z1 imaging done at various induction levels
- 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))
FACS:
- Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
- pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
- Fluorescence due to M9 solution (Control)
Analysis
Microscopy:
pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn
Red : K12Z1 Fluorescence values at different aTc induction levels
Green : pT.luxI.C Fluorescence values at different aTc induction levels
Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels
Linear and log space
gallery
