June 20
From 2007.igem.org
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'''Mean and Standard Error Plots at all induction levels''' | '''Mean and Standard Error Plots at all induction levels''' | ||
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| - | Image:CFPlin.jpg|A linear plot of mean CFP Fluorescence values at | + | Image:CFPlin.jpg|A linear plot of mean CFP Fluorescence values at all aTc concentrations.The low values of the red points show that aTc causes no CFP fluorescence in the absence of pT.luxI.C as is in K12Z1. Also, the blue and green points almost form a sigmoid, except for the value at 100 aTc. |
Image:YFPlin.jpg | Image:YFPlin.jpg | ||
Image:RFPlin.jpg | Image:RFPlin.jpg | ||
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Image:CFPlog.jpg | Image:CFPlog.jpg | ||
Revision as of 15:51, 25 June 2007
Experiments
Microscopy:
- pT luxI Cfp imaging done at various induction levels but OD was too high
- K12Z1 imaging done at various induction levels
- 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))
FACS:
- Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
- pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
- Fluorescence due to M9 solution (Control)
Analysis
Microscopy:
pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn
Red : K12Z1 Fluorescence values at different aTc induction levels
Green : pT.luxI.C Fluorescence values at different aTc induction levels
Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels
Linear and log space
Mean and Standard Error Plots at all induction levels
 A linear plot of mean CFP Fluorescence values at all aTc concentrations.The low values of the red points show that aTc causes no CFP fluorescence in the absence of pT.luxI.C as is in K12Z1. Also, the blue and green points almost form a sigmoid, except for the value at 100 aTc. |
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