June 20

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Experiments

Microscopy:

  • pT luxI Cfp imaging done at various induction levels but OD was too high
  • K12Z1 imaging done at various induction levels
  • 1st Open loop trial Experiment DONE (Auto fluorescence, pT.luxI.C (at AI OD 2 and 0.2))

FACS:

  • Auto fluorescence at inductions [0,1,10,25,50,100] done in triplets
  • pT.luxI.C fluorescence at inductions [0,1,10,25,50,100] done in triplets
  • Fluorescence due to M9 solution (Control)

Analysis

Microscopy:

pT LuxI.C: Subtract K12Z1 and plot CFP,YFP,RFP vs aTC concn

Red : K12Z1 Fluorescence values at different aTc induction levels

Green : pT.luxI.C Fluorescence values at different aTc induction levels

Blue : pT.luxI.C - K12Z1 Fluorescence values at different aTc induction levels

Linear and log space

Mean and Standard Error Plots at all induction levels