McGill/Antibiotics
From 2007.igem.org
< McGill(Difference between revisions)
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The following is the protocol for preparing Kanamycin and Ampicillin from their stock compunds:<br> | The following is the protocol for preparing Kanamycin and Ampicillin from their stock compunds:<br> | ||
#Add less than 5ml dH20 to a large volume tube (blue caps) eg. 4ml. | #Add less than 5ml dH20 to a large volume tube (blue caps) eg. 4ml. | ||
| - | #Add | + | #Add 500ug Amp stock ->100ug/ml or, 50ug Kan stock -> 10ug/ml. |
#Add water till the final volume is made up to 5ml. | #Add water till the final volume is made up to 5ml. | ||
#Filter sterilize (remember the syringe screws onto the filter to be secured, not pushed in. | #Filter sterilize (remember the syringe screws onto the filter to be secured, not pushed in. | ||
#With a P1000 pipette, extract 1ml into 5 epitubes. | #With a P1000 pipette, extract 1ml into 5 epitubes. | ||
#Store @ -20C. | #Store @ -20C. | ||
Latest revision as of 16:18, 4 July 2007
Preparing Antibiotics:
The following is the protocol for preparing Kanamycin and Ampicillin from their stock compunds:
- Add less than 5ml dH20 to a large volume tube (blue caps) eg. 4ml.
- Add 500ug Amp stock ->100ug/ml or, 50ug Kan stock -> 10ug/ml.
- Add water till the final volume is made up to 5ml.
- Filter sterilize (remember the syringe screws onto the filter to be secured, not pushed in.
- With a P1000 pipette, extract 1ml into 5 epitubes.
- Store @ -20C.
