From 2007.igem.org

October 2007

October 5

Jimmy and I midiprepped 50mL of each E0434 & I5611 using Annette's procedure. Could not prep more because 1. annette's procedure required the clinical centrigue where there are only 6 spots for 15ml tubes, 2.there were not enough sterile epi tubes, 3. could not use real Qiagen procedure instead (the rotor we needed in the big centrifuge is broken). I'm not sure about the yield of this. definitely check the dna conc before screening. one of the last steps in annette's procedure involving the pooling of pellets of DNA from 6 epi tubes to 1 tube using ethanol is iffy and there may be a lot of dna loss there.

I've lysated the pellet from another 50mL of each (w/P1) and froze it in case we want to do this again. It has green tape around it. The 2 epi tubes w/ the midi are next to it and also have green tape around them, in the top right corner of the freezer.

Horia