Mechanism I

From 2007.igem.org

Revision as of 09:31, 5 August 2007 by YFBao (Talk | contribs)

We propose to link the riboflavin operon of Bacillus subtilis to plasmid puc18-ADH1 p+t(figure1). ADH1p(560bp) is a strong promoter of Yeast and ADH1t is the terminater corresponding to this promoter. The initial carrier of puc18-ADH1 p+t is PUC18. The construction of this plamid is:

  1. Digest ADH1p with XbaI and BAMHI and link it to the relevant site of PUB18;
  2. Digest ADH1t with KpnI and SacI and link it to the relevant site of PUC18.

Our main work is :

Retrieved from "http://2007.igem.org/wiki/index.php/Mechanism_I"