Melb:Plan:AND Gate
From 2007.igem.org
(Difference between revisions)
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#Retransform c1 inverter (afternoon + overnight) | #Retransform c1 inverter (afternoon + overnight) | ||
#Liquid culture (overnight) | #Liquid culture (overnight) | ||
| - | #Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) | + | #Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day) |
| - | # | + | #Confirm Isolation and part using XbaI/PstI digest (half day) |
| + | #*If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert) | ||
| + | #If positive ligate (overnight) | ||
| + | #Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) | ||
| + | #Miniprep and digest to confirm and glycerol stocks. | ||
Revision as of 08:07, 11 August 2007
- Retransform c1 inverter (afternoon + overnight)
- Liquid culture (overnight)
- Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
- Confirm Isolation and part using XbaI/PstI digest (half day)
- If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
- If positive ligate (overnight)
- Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
- Miniprep and digest to confirm and glycerol stocks.
