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Revision as of 22:19, 8 August 2007 (view source) Patricia (Talk | contribs) ← Older edit		Latest revision as of 08:08, 11 August 2007 (view source) Patricia (Talk | contribs)
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	[[Melbourne/Plan|<return to plans page>]]		[[Melbourne/Plan|<return to plans page>]]
-	Watch this space. There will be a plan here by Saturday night - Sorry it's not here yet	+	#Retransform c1 inverter (afternoon + overnight)
		+	#Liquid culture (overnight)
		+	#Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
		+	#Confirm Isolation and part using XbaI/PstI digest (half day)
		+	#*If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
		+	#If positive ligate (overnight)
		+	#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
		+	#Miniprep and digest to confirm and glycerol stocks. (1 day)
		+
		+	Next need to ligate to RBS-ComA-Ter construct.

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Latest revision as of 08:08, 11 August 2007

<return to plans page>

1. Retransform c1 inverter (afternoon + overnight)
2. Liquid culture (overnight)
3. Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
4. Confirm Isolation and part using XbaI/PstI digest (half day)
   • If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
5. If positive ligate (overnight)
6. Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
7. Miniprep and digest to confirm and glycerol stocks. (1 day)

Next need to ligate to RBS-ComA-Ter construct.