http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&feed=atom&action=history
Melb:Plan:AND Gate - Revision history
2024-03-28T22:28:38Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&diff=14535&oldid=prev
Patricia at 08:08, 11 August 2007
2007-08-11T08:08:57Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:08, 11 August 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#If positive ligate (overnight)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#If positive ligate (overnight)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Miniprep and digest to confirm and glycerol stocks.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Miniprep and digest to confirm and glycerol stocks<ins class="diffchange diffchange-inline">. (1 day)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Next need to ligate to RBS-ComA-Ter construct</ins>.</div></td></tr>
</table>
Patricia
http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&diff=14534&oldid=prev
Patricia at 08:07, 11 August 2007
2007-08-11T08:07:17Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:07, 11 August 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Retransform c1 inverter (afternoon + overnight)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Retransform c1 inverter (afternoon + overnight)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Liquid culture (overnight)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Liquid culture (overnight)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Isolate plasmid (mini prep kit not working - to big? - best way to isolate?)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Isolate plasmid (mini prep kit not working - to big? - best way to isolate?<ins class="diffchange diffchange-inline">) (half day</ins>)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">Confirm Isolation and part using XbaI/PstI digest (half day)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#*If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#If positive ligate (overnight)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Miniprep and digest to confirm and glycerol stocks.</ins></div></td></tr>
</table>
Patricia
http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&diff=14533&oldid=prev
Patricia at 07:58, 11 August 2007
2007-08-11T07:58:40Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:58, 11 August 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Melbourne/Plan|<return to plans page>]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Melbourne/Plan|<return to plans page>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Watch this space. There will be a plan here by Saturday night - Sorry it's </del>not <del class="diffchange diffchange-inline">here yet</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Retransform c1 inverter (afternoon + overnight)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Liquid culture (overnight)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Isolate plasmid (mini prep kit </ins>not <ins class="diffchange diffchange-inline">working - to big? - best way to isolate?)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins></div></td></tr>
</table>
Patricia
http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&diff=14135&oldid=prev
Patricia at 22:19, 8 August 2007
2007-08-08T22:19:43Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:19, 8 August 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Melbourne/Plan|<return to plans page>]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Watch this space. There will be a plan here by Saturday night - Sorry it's not here yet</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Watch this space. There will be a plan here by Saturday night - Sorry it's not here yet</div></td></tr>
</table>
Patricia
http://2007.igem.org/wiki/index.php?title=Melb:Plan:AND_Gate&diff=14134&oldid=prev
Patricia at 22:18, 8 August 2007
2007-08-08T22:18:32Z
<p></p>
<p><b>New page</b></p><div>Watch this space. There will be a plan here by Saturday night - Sorry it's not here yet</div>
Patricia