Melb:Plan:AND Gate


Revision as of 08:07, 11 August 2007 by Patricia (Talk | contribs)

<return to plans page>

  1. Retransform c1 inverter (afternoon + overnight)
  2. Liquid culture (overnight)
  3. Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
  4. Confirm Isolation and part using XbaI/PstI digest (half day)
    • If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
  5. If positive ligate (overnight)
  6. Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
  7. Miniprep and digest to confirm and glycerol stocks.