Melbourne

From 2007.igem.org

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* University of Melbourne
* University of Melbourne
* Faculty of Biomedical Engineering
* Faculty of Biomedical Engineering
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* Bio21 Institute
* Bio21 Institute
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* Vice Chancellor (Research) ’s Office  
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* Vice Chancellor (Research) Office  
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Revision as of 06:57, 25 October 2007

Melbourne banner.png

The team welcomes you to the Melbourne University IGEM2007 Wiki!


"Coliforming"

Project overview (Short Description)

Background & Papers referenced

Plan

Achievements


Project This project aims to use light to form a solid fluorescent mass of E. coli where two light beams intersect in a suspension of cells. We've called our building system "Coliforming".


Motivation Possible applications in building complex scaffolds in tissue engineering.


Requirements The system requires six biological components.

  1. Red photosensor
  2. Blue photosensor
  3. AND gate
  4. GFP fluorescent reporter
  5. Promotable surface expression of cadherins
  6. Gas vesicle expression to produce neutrally bouyant bacteria


High Level System Design

  • Induce or constitutively express gas vesicles for neutral bouyancy
  • If (Induced & Red & Blue) then promote integrins, otherwise do not.


Achievements

  • Produced neutrally bouyant bacteria
  • Produced parts of other biological components required.


Procedures & Practical Matters



Sponsors

  • University of Melbourne
  • Faculty of Biomedical Engineering
  • Bio21 Institute
  • Vice Chancellor (Research) Office


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