Melbourne/BBa B0014

From 2007.igem.org

< Melbourne(Difference between revisions)
(Status)
(Expected Digestion Products)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
[[Melbourne/Parts used|<Return to Parts Used>]] [[Melbourne/Lab Notebook|<Return to Lab notebook>]] [[Melbourne|<Return to team homepage>]]
[[Melbourne/Parts used|<Return to Parts Used>]] [[Melbourne/Lab Notebook|<Return to Lab notebook>]] [[Melbourne|<Return to team homepage>]]
=Name: Double Terminator=
=Name: Double Terminator=
 +
Last Updated: 10:20am 09/07/07
==Physical DNA==
==Physical DNA==
Line 18: Line 19:
**Location: Cold Room
**Location: Cold Room
**Miniprepped: 5/7
**Miniprepped: 5/7
-
***Location: No
+
***Location: -20 freezer
-
**Confirmed: No
+
***Confirmed: Yes
-
**Glycerol Stock: No
+
**Glycerol Stock: 5/7
-
***Location: No
+
***Location: -80 freezer
==Expected Digestion Products==
==Expected Digestion Products==
 +
{| border="1"
 +
|+ Expected Digestion Products
 +
! Digest !! Fragment 1 !! Fragment 2
 +
|-
 +
! EcoRI/PstI
 +
| 131 || 3153
 +
|-
 +
! EcoRI/HaeII
 +
| 579 || 2705
 +
|}
 +
__NOTOC__
__NOTOC__

Latest revision as of 05:07, 10 July 2007

<Return to Parts Used> <Return to Lab notebook> <Return to team homepage>

Name: Double Terminator

Last Updated: 10:20am 09/07/07

Physical DNA

  • Registry: BBa_0014
  • Kit location: P1 1G
  • Resistance: Amp, Kan
  • Part Size: 95
  • Plasmid Vector: pSB1AK3
  • Vector Size: 3189
  • Total Size: 3284

Status

  • Labelling: P1 1G
  • Resuspended: Yes
    • Location: -20 freezer
  • Transformed: Yes
    • Location: Cold Room
    • Miniprepped: 5/7
      • Location: -20 freezer
      • Confirmed: Yes
    • Glycerol Stock: 5/7
      • Location: -80 freezer

Expected Digestion Products

Expected Digestion Products
Digest Fragment 1 Fragment 2
EcoRI/PstI 131 3153
EcoRI/HaeII 579 2705