Melbourne/Blue Photosensor Background

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[[Melbourne/Background|<return to top of background>]]  [[melbourne|<return to home page>]]   
[[Melbourne/Background|<return to top of background>]]  [[melbourne|<return to home page>]]   
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====Preliminaries====
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SopII - sensory rhodopsin II
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[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA Seqeunce]]
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[[Melbourne/Blue Photosensor Background/SopII/AA|AA Sequence]]
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
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HtrII - sensory rhodopsin II transducer
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[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA Seqeunce]]
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[[Melbourne/Blue Photosensor Background/SopII/AA|AA Sequence]]
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
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====References====
 
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This part is based on “Photostimulation of a Sensory Rhodopsin II/HtrII/Tsr Fusion Chimera Activates CheA-Autophosphorylation and CheY-Phosphotransfer in Vitro” by Vishwa D. Trivedi and John L. Spudich, Biochemistry 2003, 42, 13887-13892.
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SopII/HtrII fusion
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Acording to this article the peak sensitivity is to 500+/-5nm, and results in a 3 fold activation of the Tsr.. CheA,W,Y connected system.  
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[[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA Seqeunce]]
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[[Melbourne/Blue Photosensor Background/Fusion/AA|AA Sequence]]
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
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It is proposed to replace Tsr with homolgouse CopP.
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ComP - two-component sensor histidine kinase
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SRII-HtrII fusion to which CopP is fused
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[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA Seqeunce]]
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CopA when phosphorylated by CopP is an activator for PsfA promoter sequence from
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[[Melbourne/Blue Photosensor Background/SopII/AA|AA Sequence]]
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Dr Alan Grossman (M.I.T.)
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
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Based on
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*SRII-HtrII-Tsr fusion from Prof J.L. Spudich  (university of Texas)[[melb:spudich N sequence]]
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*BBa_J51000 (ComP) kinase
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*BBa_J51001 (ComA) activator
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PARTS:
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ComA - sensory rhodopsin II transducer
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* SrfA promoter
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[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA Seqeunce]]
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* ComA protein generator
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[[Melbourne/Blue Photosensor Background/SopII/AA|AA Sequence]]
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* SRII-ComP photosensor
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[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
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* Any phyco construction genes?
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SRII is from Natronomonas pharaonis.
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[[Image:Example.jpg]]
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Tsr fusion was made by Jung et al J Bacteriol 183 6365-6371 (2001) they propose a mechanism.
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I don’t see why anyone thinks this will work!!! Currently a conformational change induced by light increases affinity in TSR for Che family which leads to cross phosphorylation. To replace TSR with a kinase would require the kinase activity to be modulated – hence matching using homology as was done for tsr is not likely to work.
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Also what are the normal functions of ComP etc.
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Revision as of 10:46, 6 August 2007

<return to top of background> <return to home page>

Preliminaries

SopII - sensory rhodopsin II DNA Seqeunce AA Sequence genbank

HtrII - sensory rhodopsin II transducer DNA Seqeunce AA Sequence genbank


SopII/HtrII fusion DNA Seqeunce AA Sequence genbank

ComP - two-component sensor histidine kinase DNA Seqeunce AA Sequence genbank

ComA - sensory rhodopsin II transducer DNA Seqeunce AA Sequence genbank

Example.jpg

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