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Revision as of 05:15, 2 July 2007

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1. Aliquot 5mL LB into 50mL falcon tube.
2. Add selective antibiotic in amount required for desired concentration.
   1. Add 10uL Ampicillin Stock (5mg/mL) leads to final concentration of 100ug/mL.
   2. Add 5uL Kanamycin Stock (5mg/mL) leads to final concentration of 50ug/mL.
3. Select single colony from agar plate and introduce to LB.
4. Incubate at 37degrees with shaking for approximately 18hrs (varies depending on growth rate of cells)