Melbourne/Growing up cells
From 2007.igem.org
- Aliquot 5mL LB into 50mL falcon tube.
- Add selective antibiotic in amount required for desired concentration.
- Add 10uL Ampicillin Stock (5mg/mL) leads to final concentration of 100ug/mL.
- Add 5uL Kanamycin Stock (5mg/mL) leads to final concentration of 50ug/mL.
- Select single colony from agar plate and introduce to LB.
- Incubate at 37degrees with shaking for approximately 18hrs (varies depending on growth rate of cells)