Melbourne/Growing up cells

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  1. Aliquot 5mL LB into 50mL falcon tube.
  2. Add selective antibiotic in amount required for desired concentration.
    1. Add 10uL Ampicillin Stock (5mg/mL) leads to final concentration of 100ug/mL.
    2. Add 5uL Kanamycin Stock (5mg/mL) leads to final concentration of 50ug/mL.
  3. Select single colony from agar plate and introduce to LB.
  4. Incubate at 37degrees with shaking for approximately 18hrs (varies depending on growth rate of cells)