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	====PCR mix====		====PCR mix====
	5ul 10x buffer\\		5ul 10x buffer\\
		+
	5ul Enhancer		5ul Enhancer
	0.6ul dNTPs (25mM stock)		0.6ul dNTPs (25mM stock)

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Revision as of 14:46, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

5ul 10x buffer\\

5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM)

• 1.5ul Primer II (10uM)
• 1ul Template (pJS010)
• 0.4ul Pfx Platinum (Invitrogen)
• 32.5ul ddH₂O

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• 50ul Total