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Revision as of 14:46, 10 October 2007 (view source) Alisa (Talk | contribs) ← Older edit		Revision as of 14:47, 10 October 2007 (view source) Alisa (Talk | contribs) Newer edit →
Line 5:		Line 5:
	5ul Enhancer		5ul Enhancer
		+
	0.6ul dNTPs (25mM stock)		0.6ul dNTPs (25mM stock)
		+
	2.5ul MgSO4 (Supplied in PCR kit)		2.5ul MgSO4 (Supplied in PCR kit)
		+
	1.5ul Primer I (10uM)		1.5ul Primer I (10uM)
-	*1.5ul Primer II (10uM)
-	*1ul Template ([[Melbourne/pJS010|pJS010]])
-	*0.4ul Pfx Platinum (Invitrogen)
-	*32.5ul ddH<sub>2</sub>O
		+	1.5ul Primer II (10uM)
		+
		+	1ul Template ([[Melbourne/pJS010|pJS010]])
		+
		+	0.4ul Pfx Platinum (Invitrogen)
		+
		+	32.5ul ddH<sub>2</sub>O
	--		--
-	*50ul Total	+	50ul Total

---

Revision as of 14:47, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH₂O -- 50ul Total