Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

(Difference between revisions)
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32.5ul ddH<sub>2</sub>O
32.5ul ddH<sub>2</sub>O
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50ul Total
50ul Total

Revision as of 14:47, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH2O

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50ul Total