Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

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=Protocol for PCR reactions A~G=  
=Protocol for PCR reactions A~G=  
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;"
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|-
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! PCR mix
 +
! PCR program
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|-
 +
|5ul 10x buffer\\
 +
 +
5ul Enhancer
 +
 +
0.6ul dNTPs (25mM stock)
 +
 +
2.5ul MgSO4 (Supplied in PCR kit)
 +
 +
1.5ul Primer I (10uM)
 +
 +
1.5ul Primer II (10uM)
 +
 +
1ul Template ([[Melbourne/pJS010|pJS010]])
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 +
0.4ul Pfx Platinum (Invitrogen)
 +
 +
32.5ul ddH<sub>2</sub>O
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|-
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| <code><nowiki><math>\sqrt{2}</math></nowiki></code>
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| <math>\sqrt{2}</math>
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| <code><nowiki>&amp;radic;2</nowiki></code>
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| &radic;2
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|-
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| <code><nowiki><math>\sqrt{1-e^2}</math></nowiki></code>
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| <math>\sqrt{1-e^2}</math>
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| <code><nowiki>&amp;radic;(1&amp;minus;''e''&amp;sup2;)</nowiki></code>
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| &radic;(1&minus;''e''&sup2;)
 +
|}
|-
|-

Revision as of 14:54, 10 October 2007

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix PCR program
5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH2O

<math>\sqrt{2}</math> <math>\sqrt{2}</math> &radic;2 √2
<math>\sqrt{1-e^2}</math> <math>\sqrt{1-e^2}</math> &radic;(1&minus;''e''&sup2;) √(1−e²)

|-

PCR mix

|5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH2O

---

50ul Total | |-

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