Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

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| √2
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| <code><nowiki><math>\sqrt{1-e^2}</math></nowiki></code>
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| 50ul Total
| <math>\sqrt{1-e^2}</math>
| <math>\sqrt{1-e^2}</math>
| <code><nowiki>&amp;radic;(1&amp;minus;''e''&amp;sup2;)</nowiki></code>
| <code><nowiki>&amp;radic;(1&amp;minus;''e''&amp;sup2;)</nowiki></code>

Revision as of 14:55, 10 October 2007

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix PCR program
5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH2O

<math>\sqrt{2}</math> <math>\sqrt{2}</math> &radic;2 √2
50ul Total <math>\sqrt{1-e^2}</math> &radic;(1&minus;''e''&sup2;) √(1−e²)

|-

PCR mix

|5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH2O

---

50ul Total | |-