Melbourne/Lab BL Notebook/PCR1

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Protocol for PCR reactions A-G (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

5ul 10x buffer 5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH<math>_{2}</math>

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